Submitted on August 14, 2008
Revised on June 8, 2009
Accepted on June 9, 2009
Parent-of-origin effects on cardiac response to pressure overload in mice
Cordelia J Barrick1, Anping Dong, Rebekah Waikel, Drew Corn1, Fanmuyi Yang, David W Threadgill, and Susan S Smyth2*
1 University of North Carolina
2 University of Kentucky
* To whom correspondence should be addressed. E-mail: susansmyth{at}uky.edu.
Left ventricular hypertrophy (LVH) is an independent risk factor for cardiovascular mortality and is commonly caused by hypertension. In rodents, transverse aortic constriction (TAC) is a model regularly employed in mechanistic studies of the response of the LV to pressure overload. We previously reported that inbred strains of male mice manifest different cardiac responses to TAC, with C57BL/6J (B6) developing LV dilatation and impaired contractility and 129S1/SvImJ (129) males displaying concentric LVH. In the present study, we investigated gender and parent-of-origin effects on the response to TAC by comparing cardiac function, organ weights, expression of cardiac hypertrophy markers, and histology in female B6 and female 129 mice and in F1 progeny of reciprocal crosses between B6 and 129 mice (B6129F1 and 129B6F1). Five weeks after TAC, heart weight increased to the greatest extent in 129B6F1 mice and the least extent in 129 and B6129F1 mice. Female 129B6F1 and B6 mice were relatively protected from the increase in heart weight that occurs in their male counterparts with pressure overload. The response to TAC in 129 consomic mice bearing the B6 Y-chromosome resembled that of 129 rather than 129B6F1 mice, indicating that the B6Y chromosome does not account for the differences in the reciprocal cross. Our results suggest that susceptibility to LVH is more complex than simple Mendelian inheritance and that parental-origin effects strongly impact the LV response to TAC in these commonly used inbred strains.
