Submitted on November 14, 2008
Revised on June 24, 2009
Accepted on June 24, 2009
Novel strategy for measuring creatine kinase reaction rate in the in vivo heart
Qiang Xiong1, Qinglu Li1, Abdul Mansoor, Mohammad Nurulqadr Jameel1, Fei Du1, Wei Chen1, and Jianyi Zhang1*
1 University of Minnesota
* To whom correspondence should be addressed. E-mail: zhang047{at}umn.edu.
In the heart, the creatine kinase (CK) system plays an important role in the cascade of ATP production, transportation and utilization. The forward pseudo-first-order rate constant (kf ) for the CK reaction can be measured non-invasively by the 31P magnetic resonance spectroscopy (MRS) magnetization saturation transfer (MST) techniques. However, the measurement of MST in the in vivo heart is limited by the lengthy data acquisition time, especially for studies requiring spatial localization. This technical report presents a new method for measuring ATP production rate via creatine kinase that can reduce the MST data acquisition time by 82%. This method is validated using an in vivo pig model to evaluate the kf of myocardial CK reaction non-invasively.
