AJP - Heart and Circulatory Physiology, Vol 243, Issue 5 708-H712, Copyright © 1982 by American Physiological Society
Effect of serum and stirring on diffusive 125I-albumin and Evans blue dye uptake
D. L. Fry, G. W. Melchior and J. Mitschelen
The diffusive in vitro uptake of homologous 125I-albumin (MA, nmol.cm-2)
and Evans blue dye (EBD) (ME, nmol.cm-2) by the deendothelialized canine
aorta from serum and from a simple albumin solution with and without EBD
and with and without vigorous stirring was measured in 18 preparations. The
results show that 1) MA and ME were significantly smaller from serum than
from a simple albumin solution, 2) vigorous stirring of the liquid phase
caused a slight decrease (approximately 5%) in MA and increase
(approximately 9%) in ME, 3) MA was not influenced by the presence of EBD,
and 4) at least 90% of the radioactivity in the tissue was free
125I-albumin with an electrophoretic mobility identical to its nonlabeled
cohort molecules and albumin in the original reagent. These observations
confirm the identity of the tissue radioactivity with the labeled protein
in the reagent, show that less than 10% of the labeled protein is
irreversibly bound in the tissue, indicate that significant concentration
gradients do not occur in the reagent phase, and indicate that albumin
appears to interact with other plasma components in the reagent phase.
