AJP - Heart and Circulatory Physiology, Vol 244, Issue 3 357-H361, Copyright © 1983 by American Physiological Society
Effect of antiplatelet antibody on platelet shape change, volume, and morphology
R. W. Colman, V. T. Nachmias, D. B. Cines and A. D. Schreiber
We extended our studies of the effect of antibody on human platelets.
Monomeric immunoglobulin G (IgG) (rabbit) antiplatelet antibody was
interacted with human platelets in the absence of complement and the early
morphological changes monitored. Platelet shape change, assessed by a
rheooptical approach, was noted within 20 s and was proportional to
antibody concentration. Antiplatelet antibody-induced shape change was also
accompanied by a change in apparent platelet volume, as measured in the
resistive particle counter. Scanning and transmission electron microscopy
confirmed a disk-to-sphere transformation associated with the formation of
bulbous, short surface projections or pseudopodia. In contrast, ADP-induced
disk-to-sphere transformation was associated with the development of long,
thin filopodia emanating from the platelet. These specific effects of
antiplatelet antibody may result in altered platelet function.
