AJP - Heart AJP: Renal Physiology
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Am J Physiol Heart Circ Physiol 246: H491-H499, 1984;
0363-6135/84 $5.00
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AJP - Heart and Circulatory Physiology, Vol 246, Issue 4 491-H499, Copyright © 1984 by American Physiological Society

ARTICLES

In situ double-beam NADH laser fluorimetry: choice of a reference wavelength

G. Renault, E. Raynal, M. Sinet, M. Muffat-Joly, J. P. Berthier, J. Cornillault, B. Godard and J. J. Pocidalo

A double-beam laser fluorimeter, using a single optical fiber to guide the lights, was constructed for in situ and on-line monitoring of NADH concentration [( NADH]) from normally blood-perfused living tissues. The device was tested on an isolated blood-perfused rat heart system to determine the most efficient reference wavelength for the compensation of the hemodynamic artifact induced by blood circulation in the tissues on the fluorescence measure; 586 nm was found to be an accurate reference wavelength, and a mathematical relationship was established that allowed the digital treatment of the measured fluorescence to give a signal (compensated fluorescence) that varied only with [NADH] in the volume of tissue investigated.




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