AJP - Heart and Circulatory Physiology, Vol 247, Issue 1 35-H39, Copyright © 1984 by American Physiological Society
Role of site of microsphere injection and catheter position on systemic and regional hemodynamics in rat
I. Kobrin, M. B. Kardon, W. Oigman, B. L. Pegram and E. D. Frohlich
The influences of the site of microsphere injection (intra-atrial vs.
intraventricular) and positioning of the left ventricular catheter
(aortoventricular vs. atrioventricular) on systemic, renal, and coronary
hemodynamics were evaluated in anesthetized rats. The effect of anesthesia
on aortoventricular catheter positioning was also evaluated. In
anesthetized and open-chest preparations, the systemic and renal
hemodynamics were not affected by catheter position or site of microsphere
injection; however, myocardial blood flow was dependent on these variables.
Variations in coronary blood flow were significantly greater when the
catheter was in the aortoventricular position (34 +/- 3%) than with an
atrioventricular catheter (11 +/- 2%, P less than 0.01), irrespective of
whether the microspheres were injected into the atrium or ventricle.
Comparison of anesthetized and conscious rats with aortoventricular
catheter indicated lesser variability in coronary blood flow in the
conscious rats (P less than 0.01). Therefore, the greater variability of
coronary flow measurements in anesthetized rats was caused by the position
of the cardiac catheter in the aortoventricular route. However, the
variability caused by the aortoventricular catheter was much less in
conscious rats. Therefore, coronary flow hemodynamic measurements
(microsphere technique) are less variable when they are made in conscious
rats.
