AJP - Heart and Circulatory Physiology, Vol 253, Issue 1 107-H114, Copyright © 1987 by American Physiological Society

ARTICLES

Substrate use in ischemic and reperfused canine myocardium: quantitative considerations

D. W. Myears, B. E. Sobel and S. R. Bergmann

The patterns of use of substrate in reperfused myocardium are not yet well elucidated, and their delineation is essential for adequate interpretation of results of analyses performed after positron emission tomography with labeled substrates to differentiate normal from abnormal heart muscle. Accordingly, in open-chest, anesthetized dogs we measured glucose and fatty acid utilization in normal, ischemic, and reperfused myocardium and assessed the contributions of metabolism of each substrate to overall oxidative metabolism. Intracoronary [3H]glucose and [14C]palmitate were administered in five control dogs, eight dogs subjected to 1 h of coronary occlusion, and nine dogs subjected to reperfusion after 1 h of ischemia. Regional coronary venous blood samples were assayed sequentially. In reperfused myocardium, utilization of glucose (463 +/- 88 nmol X g-1 X min-1) was 103% greater than that in ischemic and 273% greater than in normal myocardium (P less than 0.05 for each). Utilization of fatty acid during reperfusion (55 +/- 10 nmol X g-1 X min-1), although greater than that in ischemic myocardium, was significantly less than that in normal myocardium (48% of control, P less than 0.05) despite restoration of flow to 80% of control values. Although glucose constituted 70% of the substrate oxidized in ischemic myocardium, its contribution to overall oxidative metabolism in reperfused myocardium was only 25%. In contrast, despite diminished net uptake of fatty acid, oxidation of fatty acid accounted for 63% of total oxygen consumption in reperfused myocardium. These studies indicate that canine myocardium reperfused after 1 h of ischemia exhibits enhanced uptake of glucose and impaired utilization of palmitate.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				I. C. Okere, T. A. McElfresh, D. Z. Brunengraber, W. Martini, J. P. Sterk, H. Huang, M. P. Chandler, H. Brunengraber, and W. C. Stanley Differential effects of heptanoate and hexanoate on myocardial citric acid cycle intermediates following ischemia-reperfusion J Appl Physiol, January 1, 2006; 100(1): 76 - 82. [Abstract] [Full Text] [PDF]

				M. Khairallah, F. Labarthe, B. Bouchard, G. Danialou, B. J. Petrof, and C. Des Rosiers Profiling substrate fluxes in the isolated working mouse heart using 13C-labeled substrates: focusing on the origin and fate of pyruvate and citrate carbons Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1461 - H1470. [Abstract] [Full Text] [PDF]

				M. C. Moore, S. Cardin, D. S. Edgerton, B. Farmer, D. W. Neal, M. Lautz, and A. D. Cherrington Unlike mice, dogs exhibit effective glucoregulation during low-dose portal and peripheral glucose infusion Am J Physiol Endocrinol Metab, February 1, 2004; 286(2): E226 - E233. [Abstract] [Full Text] [PDF]

				C.J. Zuurbier Postischemic Myocardial Metabolism Seminars in Cardiothoracic and Vascular Anesthesia, March 1, 2003; 7(1): 59 - 65. [PDF]

				J. Terrand, I. Papageorgiou, N. Rosenblatt-Velin, and R. Lerch Calcium-mediated activation of pyruvate dehydrogenase in severely injured postischemic myocardium Am J Physiol Heart Circ Physiol, August 1, 2001; 281(2): H722 - H730. [Abstract] [Full Text] [PDF]

				W.-S. Richter, S. Fischer, N. Ernst, and D. L. Munz Extraction of Long-Chain Fatty Acids in Isolated Rat Heart During Acute Low-Flow Ischemia J. Nucl. Med., July 1, 2001; 42(7): 1101 - 1108. [Abstract] [Full Text] [PDF]

				P. H. McNulty, D. Jagasia, G. W. Cline, C. K. Ng, J. M. Whiting, P. Garg, G. I. Shulman, and R. Soufer Persistent Changes in Myocardial Glucose Metabolism In Vivo During Reperfusion of a Limited-Duration Coronary Occlusion Circulation, February 29, 2000; 101(8): 917 - 922. [Abstract] [Full Text] [PDF]

				D. D. Belke, T. S. Larsen, G. D. Lopaschuk, and D. L. Severson Glucose and fatty acid metabolism in the isolated working mouse heart Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1999; 277(4): R1210 - R1217. [Abstract] [Full Text] [PDF]

				H. G. Pietersen, C. J. M. Langenberg, G. Geskes, A. Kester, S. d. Lange, G. J. Van der Vusse, A. J. M. Wagenmakers, and P. B. Soeters MYOCARDIAL SUBSTRATE UPTAKE AND OXIDATION DURING AND AFTER ROUTINECARDIAC SURGERY J. Thorac. Cardiovasc. Surg., July 1, 1999; 118(1): 71 - 81. [Abstract] [Full Text] [PDF]

				Y. Yamane, N. Ishide, Y. Kagaya, D. Takeyama, N. Shiba, M. Chida, T. Nozaki, T. Takahashi, T. Ido, and K. Shirato Heterogeneous fatty acid uptake early after reperfusion in rat hearts Am J Physiol Heart Circ Physiol, March 1, 1998; 274(3): H923 - H929. [Abstract] [Full Text] [PDF]