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Am J Physiol Heart Circ Physiol 254: H425-H437, 1988;
0363-6135/88 $5.00
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AJP - Heart and Circulatory Physiology, Vol 254, Issue 3 425-H437, Copyright © 1988 by American Physiological Society

ARTICLES

Specific albumin binding to microvascular endothelium in culture

J. E. Schnitzer, W. W. Carley and G. E. Palade
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.

The specific binding of rat serum albumin (RSA) to confluent microvascular endothelial cells in culture derived from the vasculature of the rat epididymal fat pad was studied at 4 degrees C by radioassay and immunocytochemistry. Radioiodinated RSA (125I-RSA) binding to the cells reached equilibrium at approximately 20 min incubation. Albumin binding was a slowly saturating function over concentrations ranging from 0.01 to 50 mg/ml. Specific RSA binding with a moderate apparent affinity constant of 1.0 mg/ml and with a maximum binding concentration of 90 ng/cm2 was immunolocalized with anti-RSA antibody to the outer (free) side of the endothelium. Scatchard analysis of the binding yielded a nonlinear binding curve with a concave-upward shape. Dissociation rate analysis supports negative cooperativity of albumin binding, but multiple binding sites may also be present. Albumin binding fulfilled many requirements for ligand specificity including saturability, reversibility, competibility, and dependence on both cell type and cell number. The results are discussed in terms of past in situ investigations on the localization of albumin binding to vascular endothelium and its effect on transendothelial molecular transport.


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