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Am J Physiol Heart Circ Physiol 256: H1255-H1260, 1989;
0363-6135/89 $5.00
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AJP - Heart and Circulatory Physiology, Vol 256, Issue 4 1255-H1260, Copyright © 1989 by American Physiological Society

ARTICLES

Laser-Doppler assessment of brain microcirculation: effect of local alterations

R. L. Haberl, M. L. Heizer and E. F. Ellis
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.

The objective of this study was to determine whether changes in blood perfusion measured by laser-Doppler (LD) flowmetry correlate with simultaneously measured changes in pial arteriolar diameter after local application of vasoactive agents on the brain surface. A closed cranial window was implanted in anesthetized rabbits. A 0.84-mm-diam LD probe was inserted through one window port and fixed at a distance of 1-2 mm from the cortical surface. The probe detects changes in perfusion within a tissue volume of approximately 1 mm3. The diameter of pial arterioles in the area adjacent to the LD probe was simultaneously measured with a microscope and image-splitting device. Topical application of bradykinin (80 nM to 8 microM), which stimulates the formation of dilator prostaglandins and O2 radicals, induced a dose-dependent arteriolar dilation and increase in LD flow. Topical application of 33 microM 2-chloroadenosine, a stable analogue of adenosine, induced the same degree of pial arteriolar dilation as 8 microM bradykinin but produced a much larger increase in LD flow, probably due to its greater tissue penetration and stability. At 5 min after bradykinin washout the arterioles had nearly returned to their control diameter, whereas LD flow was still increased. Similarly, there was a discrepancy between LD flow and diameter changes after washout of 2-chloroadenosine. We conclude that LD flowmetry is a useful technique for continuous assessment of cortical blood flow in response to topically applied agents.(ABSTRACT TRUNCATED AT 250 WORDS)


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