AJP - Heart Myographs and Tissue organ baths
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 257: H280-H288, 1989;
0363-6135/89 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Austin, R. E.
Right arrow Articles by Hoffman, J. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Austin, R. E., Jr
Right arrow Articles by Hoffman, J. I.

AJP - Heart and Circulatory Physiology, Vol 257, Issue 1 280-H288, Copyright © 1989 by American Physiological Society

ARTICLES

Quantitating error in blood flow measurements with radioactive microspheres

R. E. Austin Jr, W. W. Hauck, G. S. Aldea, A. E. Flynn, D. L. Coggins and J. I. Hoffman
Cardiovascular Research Institute, University of California, San Francisco 94143.

Accurate determination of the reproducibility of measurements using the microsphere technique is important in assessing differences in blood flow to different organs or regions within organs, as well as changes in perfusion under various experimental conditions. The sources of error of the technique are briefly reviewed. In addition, we derived a method for combining quantifiable sources of error into a single estimate that was evaluated experimentally by simultaneously injecting eight or nine sets of microspheres (each with a different radionuclide label) into four anesthetized dogs. Each nuclide was used to calculate blood flow in 145-190 myocardial regions. We compared each flow determination (using a single nuclide label) with a weighted mean for the piece (based on the remaining nuclides). The difference was defined as "measured" error. In all, there were a total of 5,975 flow observations. We compared measured error with theoretical estimates based on the Poisson error of radioactive disintegration and microsphere entrapment, nuclide separation error, and reference flow error. We found that combined estimates based on these sources completely accounted for measured error in the relative distribution of microspheres. In addition, our estimates of the error in measuring absolute flows (which were established using microsphere reference samples) slightly, but significantly, underestimated measured error in absolute flow.


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
H. Anetzberger, E. Thein, M. Becker, A. K. Walli, and K. Messmer
Validity of fluorescent microspheres method for bone blood flow measurement during intentional arterial hypotension
J Appl Physiol, September 1, 2003; 95(3): 1153 - 1158.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. C. Marshall, P. Powers-Risius, B. W. Reutter, A. M. Schustz, C. Kuo, M. K. Huesman, and R. H. Huesman
Flow heterogeneity following global no-flow ischemia in isolated rabbit heart
Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H654 - H667.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. S. Aldea, H. Mori, W. K. Husseini, R. E. Austin, and J. I. E. Hoffman
Effects of increased pressure inside or outside ventricles on total and regional myocardial blood flow
Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H2927 - H2938.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
W. A. Altemeier, S. McKinney, and R. W. Glenny
Fractal nature of regional ventilation distribution
J Appl Physiol, May 1, 2000; 88(5): 1551 - 1557.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Hubler, J. E. Souders, E. D. Shade, M. P. Hlastala, N. L. Polissar, and R. W. Glenny
Validation of fluorescent-labeled microspheres for measurement of relative blood flow in severely injured lungs
J Appl Physiol, December 1, 1999; 87(6): 2381 - 2385.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. C. Becker, R. W. Jeremy, J. Schaper, and W. Schaper
Ultrastructural assessment of myocardial necrosis occurring during ischemia and 3-h reperfusion in the dog
Am J Physiol Heart Circ Physiol, July 1, 1999; 277(1): H243 - H252.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. S. Gross, A. A. Damji, S. Judex, R. C. Bray, and R. F. Zernicke
Bone hyperemia precedes disuse-induced intracortical bone resorption
J Appl Physiol, January 1, 1999; 86(1): 230 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. X. Mazoit, R. Le Guen, A. Decaux, P. Albaladejo, and K. Samii
Application of HPLC to counting of colored microspheres in determination of regional blood flow
Am J Physiol Heart Circ Physiol, March 1, 1998; 274(3): H1041 - H1047.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. F. Buchwalder, M. Lin, T. J. McDonald, and P. W. Nathanielsz
Fetal sheep adrenal blood flow responses to hypoxemia after splanchnicotomy using fluorescent microspheres
J Appl Physiol, January 1, 1998; 84(1): 82 - 89.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online