AJP - Heart and Circulatory Physiology, Vol 257, Issue 2 525-H533, Copyright © 1989 by American Physiological Society

ARTICLES

Influence of saline infusion on blood-tissue albumin transport

E. M. Renkin, K. Rew, M. Wong, D. O'Loughlin and L. Sibley
Department of Human Physiology, School of Medicine, University of California, Davis 95616.

Anesthetized rats were infused with lactated Ringer solution (LR) at constant rate for 30 or 60 min; delivered volume loads ranged from 0.03 to 0.08 ml/g body wt. Controls were given only a sustaining infusion of saline at 0.002 ml.g-1.h-1. Only 7-14% of the LR remained in the plasma at the end of the infusion; 76-88% entered the interstitial compartment, and 7-17% was excreted. The amount of plasma protein lost from the circulation with the extravasated fluid was studied simultaneously by two methods: 1) material balance in the whole animal and 2) changes in 131I-labeled albumin uptake (VA) and water content (VW) in individual tissues. The extravasation of 0.03-0.06 ml fluid/g body wt (75-160% initial plasma volume) did not significantly increase plasma protein extravasation in the whole rat. Nearly all of the sampled tissues of LR-infused rats had higher VW than controls. Tissue VA tended to increase with VW, but the regression slopes (delta VA/delta VW), a measure of the tracer albumin concentration of capillary filtrate relative to plasma, were low; skin, 0.006; paw, 0.018; skeletal muscles, 0.007; heart, 0.057; jejunum, 0.095; ileum, 0.045; cecum, 0.026; and colon, 0.027. These ratios are consistent with the very small loss of total plasma protein observed and attest to high solvent-drag reflection coefficients (sigma approximately equal to 1 - delta VA/delta VW): greater than 0.98 in capillaries of skeletal muscles, skin, and paw and 0.91-0.97 in heart and intestine.

This article has been cited by other articles:

				B. A. Borge, V. V. Iversen, and R. K. Reed Changes in plasma protein extravasation in rat skin during inflammatory challenges evaluated by microdialysis Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H2108 - H2115. [Abstract] [Full Text] [PDF]

				K. Sampei, J. A. Ulatowski, Y. Asano, H. Kwansa, E. Bucci, and R. C. Koehler Role of nitric oxide scavenging in vascular response to cell-free hemoglobin transfusion Am J Physiol Heart Circ Physiol, September 1, 2005; 289(3): H1191 - H1201. [Abstract] [Full Text] [PDF]

				J. A. Russell, C. A. Kindig, B. J. Behnke, D. C. Poole, and T. I. Musch Effects of aging on capillary geometry and hemodynamics in rat spinotrapezius muscle Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H251 - H258. [Abstract] [Full Text] [PDF]

				K. R. Olson, D. W. Kinney, R. A. Dombkowski, and D. W. Duff Transvascular and intravascular fluid transport in the rainbow trout: revisiting Starling's forces, the secondary circulation and interstitial compliance J. Exp. Biol., February 1, 2003; 206(3): 457 - 467. [Abstract] [Full Text] [PDF]

				B. W. Oqvist, A. Rippe, J. Tencer, and B. Rippe In vivo microvascular clearance of albumin in renal and extrarenal tissues in puromycin aminonucleoside (PAN) induced nephrotic syndrome Nephrol. Dial. Transplant., August 1, 1999; 14(8): 1898 - 1903. [Abstract] [Full Text] [PDF]