AJP - Heart and Circulatory Physiology, Vol 258, Issue 2 285-H291, Copyright © 1990 by American Physiological Society

ARTICLES

Sarcolemmal integrity and metabolic competence of cardiomyocytes under anoxia-reoxygenation

B. Siegmund, A. Koop, T. Klietz, P. Schwartz and H. M. Piper
Physiologisches Institut I, Universitat Dusseldorf, Federal Republic of Germany.

In tissue, mechanical cell-to-cell interactions may contribute to cardiomyocyte injury in anoxia-reoxygenation. In the present study, the disturbance of energy metabolism and cell injury were investigated in isolated cardiomyocytes, free of external mechanical constraints. Cardiomyocytes from adult rat, attached to culture dishes, were exposed to 120 min of anoxia and 15 min of reoxygenation in a substrate-free modified Tyrode solution. The energetic state of the cells in anoxia-reoxygenation was characterized by the free-energy change of ATP hydrolysis (delta GATP), amounting to 57 kJ/mol ATP in normoxia. After 120 min of anoxia, all cells were contracted to 65% of their length and delta GATP decreased to 41 kJ/mol. No lactate dehydrogenase was released. Reoxygenation caused a partial oxygen paradox: immediate hypercontracture of the cells, but no release of lactate dehydrogenase. delta GATP recovered to 51 kJ/mol within 15 min. The results demonstrate that anoxic cardiomyocytes can be energy depleted without losing sarcolemmal integrity. They can undergo hypercontracture, elicited by reoxygenation, and yet an almost normal delta GATP can be reestablished.

This article has been cited by other articles:

				N. Maulik Effect of p38 MAP kinase on cellular events during ischemia and reperfusion: possible therapy Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2302 - H2303. [Full Text] [PDF]

				M. Kido, H. Otani, S. Kyoi, T. Sumida, H. Fujiwara, T. Okada, and H. Imamura Ischemic preconditioning-mediated restoration of membrane dystrophin during reperfusion correlates with protection against contraction-induced myocardial injury Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H81 - H90. [Abstract] [Full Text] [PDF]

				B. N. Eigel and R. W. Hadley Antisense inhibition of Na+/Ca2+ exchange during anoxia/reoxygenation in ventricular myocytes Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H2184 - H2190. [Abstract] [Full Text] [PDF]

				C. Schafer, Y. V. Ladilov, B. Siegmund, and H. M. Piper Importance of bicarbonate transport for protection of cardiomyocytes against reoxygenation injury Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1457 - H1463. [Abstract] [Full Text] [PDF]

				J. Inserte, G. Taimor, B. Hofstaetter, D. Garcia-Dorado, and H. M. Piper Influence of simulated ischemia on apoptosis induction by oxidative stress in adult cardiomyocytes of rats Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H94 - H99. [Abstract] [Full Text] [PDF]

				H. M. Piper and D. Garcia-Dorado Prime causes of rapid cardiomyocyte death during reperfusion Ann. Thorac. Surg., November 1, 1999; 68(5): 1913 - 1919. [Abstract] [Full Text] [PDF]

				M. Ruiz-Meana, D. Garcia-Dorado, B. Hofstaetter, H. M. Piper, and J. Soler-Soler Propagation of Cardiomyocyte Hypercontracture by Passage of Na+ Through Gap Junctions Circ. Res., August 6, 1999; 85(3): 280 - 287. [Abstract] [Full Text] [PDF]

				L. Agullo, D. Garcia-Dorado, J. Inserte, A. Paniagua, P. Pyrhonen, J. Llevadot, and J. Soler-Soler L-Arginine limits myocardial cell death secondary to hypoxia-reoxygenation by a cGMP-dependent mechanism Am J Physiol Heart Circ Physiol, May 1, 1999; 276(5): H1574 - H1580. [Abstract] [Full Text] [PDF]

				J. A. Post, S.-Y. Wang, and G. A. Langer pHe, [Ca2+]e, and cell death during metabolic inhibition: role of phospholipase A2 and sarcolemmal phospholipids Am J Physiol Heart Circ Physiol, January 1, 1998; 274(1): H18 - H26. [Abstract] [Full Text] [PDF]

				B. Siegmund, W. Schlack, Y. V. Ladilov, C. Balser, and H. M. Piper Halothane Protects Cardiomyocytes Against Reoxygenation-Induced Hypercontracture Circulation, December 16, 1997; 96(12): 4372 - 4379. [Abstract] [Full Text]

				Y.V. Ladilov, B. Siegmund, C. Balser, and H.M. Piper Simulated Ischemia Increases the Susceptibility of Rat Cardiomyocytes to Hypercontracture Circ. Res., January 1, 1997; 80(1): 69 - 75. [Abstract] [Full Text]