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Am J Physiol Heart Circ Physiol 260: H917-H926, 1991;
0363-6135/91 $5.00
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AJP - Heart and Circulatory Physiology, Vol 260, Issue 3 917-H926, Copyright © 1991 by American Physiological Society

ARTICLES

Adenosine formation and energy status during hypoperfusion and 2-deoxyglucose infusion

M. X. He, M. W. Gorman, G. D. Romig, R. A. Meyer and H. V. Sparks Jr
Department of Physiology, Michigan State University, East Lansing 48824.

The relationship between adenosine (Ado) formation and cytosolic energy status was studied in isolated guinea pig hearts during hypoperfusion plus norepinephrine infusion (0.6 nmol/min) and in isolated rat hearts during 2-deoxyglucose (2-DG) infusion. 31P nuclear magnetic resonance (31P-NMR) was used to measure phosphate concentrations, and both phosphorylation potential (expressed as [ATP]/[ADP][Pi]) and energy charge [expressed as (([ATP] + 1/2[ADP])/([ATP] + [ADP] + [AMP]))] were calculated as indexes of cytosolic energy status. Both progressive flow reductions and increasing length of exposure to 2-DG led to progressive decreases in energy charge and phosphorylation potential. In both cases, steady-state Ado release first increased then declined despite a continued fall in energy status. Inosine release followed a similar pattern. This biphasic pattern of Ado release vs. energy charge is similar to the pattern seen in in vitro studies of cytosolic 5'-nucleotidase, supporting the hypothesis that Ado formation in vivo is regulated by the influence of energy status on this enzyme. However, Ado release in vivo peaked at an energy charge much higher (0.997) than that observed in vitro (0.60-0.86). It is therefore probable that the inhibition of Ado formation in the perfused heart occurs via factor(s) in addition to energy charge.


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