AJP - Heart and Circulatory Physiology, Vol 265, Issue 2 725-H733, Copyright © 1993 by American Physiological Society

ARTICLES

Plasmalemmal vesicles represent the large pore system of continuous microvascular endothelium

D. Predescu and G. E. Palade
Division of Cellular and Molecular Medicine, University of California, San Diego, La Jolla 92093-0651.

In the capillary physiology literature, molecules and particles larger than 10 nm are assumed to leave the plasma mostly through large pores located at the level of intercellular junctions in microvessels lined with a continuous endothelium. In morphological studies of similar microvessels, outgoing particles > 10 nm were detected in endothelial plasmalemmal vesicles not in intercellular junctions. Because the probes may not be found in transit through the junctions because they may be swept away by strong currents generated by Starling forces, we have examined a large number of junctions in arteriolar, capillary, and venular segments of bipolar vascular fields of mouse diaphragms collected after perfusion with large pore probes. The results presented in this study indicate that 1) the perfused probes accumulate in the luminal introits of the junctions as filtration residues that decrease in size and frequency from arterioles to venules, and 2) large pore probes move across the endothelium exclusively through plasmalemmal vesicles.

This article has been cited by other articles:

				S. A. Predescu, D. N. Predescu, and A. B. Malik Molecular determinants of endothelial transcytosis and their role in endothelial permeability Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L823 - L842. [Abstract] [Full Text] [PDF]

				B.-I. Rosengren, A. Rippe, C. Rippe, D. Venturoli, K. Sward, and B. Rippe Transvascular protein transport in mice lacking endothelial caveolae Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1371 - H1377. [Abstract] [Full Text] [PDF]

				D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]

				X. Yao and C. J. Garland Recent Developments in Vascular Endothelial Cell Transient Receptor Potential Channels Circ. Res., October 28, 2005; 97(9): 853 - 863. [Abstract] [Full Text] [PDF]

				D. Predescu, S. M. Vogel, and A. B. Malik Functional and morphological studies of protein transcytosis in continuous endothelia Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L895 - L901. [Abstract] [Full Text] [PDF]

				R. D. Minshall, W. C. Sessa, R. V. Stan, R. G. W. Anderson, and A. B. Malik Caveolin regulation of endothelial function Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1179 - L1183. [Abstract] [Full Text] [PDF]

				T. A. John, S. M. Vogel, C. Tiruppathi, A. B. Malik, and R. D. Minshall Quantitative analysis of albumin uptake and transport in the rat microvessel endothelial monolayer Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L187 - L196. [Abstract] [Full Text] [PDF]

				O. Carlsson, B.-I. Rosengren, and B. Rippe Transcytosis inhibitor N-ethylmaleimide increases microvascular permeability in rat muscle Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1728 - H1733. [Abstract] [Full Text] [PDF]

				S. A. Predescu, D. N. Predescu, and G. E. Palade Endothelial Transcytotic Machinery Involves Supramolecular Protein-Lipid Complexes Mol. Biol. Cell, April 1, 2001; 12(4): 1019 - 1033. [Abstract] [Full Text]

				T. Stevens, J. G. N. Garcia, D. M. Shasby, J. Bhattacharya, and A. B. Malik Mechanisms regulating endothelial cell barrier function Am J Physiol Lung Cell Mol Physiol, September 1, 2000; 279(3): L419 - L422. [Abstract] [Full Text] [PDF]

				R.-V. Stan, L. Ghitescu, B. S. Jacobson, and G. E. Palade Isolation, Cloning, and Localization of Rat PV-1, a Novel Endothelial Caveolar Protein J. Cell Biol., June 14, 1999; 145(6): 1189 - 1198. [Abstract] [Full Text] [PDF]

				D. Predescu, S. Predescu, T. McQuistan, and G. E. Palade Transcytosis of alpha 1-acidic glycoprotein in the continuous microvascular endothelium PNAS, May 26, 1998; 95(11): 6175 - 6180. [Abstract] [Full Text] [PDF]

				W. Schubert, P. G. Frank, B. Razani, D. S. Park, C.-W. Chow, and M. P. Lisanti Caveolae-deficient Endothelial Cells Show Defects in the Uptake and Transport of Albumin in Vivo J. Biol. Chem., December 21, 2001; 276(52): 48619 - 48622. [Abstract] [Full Text] [PDF]