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Am J Physiol Heart Circ Physiol 265: H741-H746, 1993;
0363-6135/93 $5.00
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AJP - Heart and Circulatory Physiology, Vol 265, Issue 2 741-H746, Copyright © 1993 by American Physiological Society

ARTICLES

Free cytosolic Ca2+ measured with Ca(2+)-selective electrodes and fura 2 in rat mesenteric resistance arteries

P. E. Jensen, M. J. Mulvany, C. Aalkjaer, H. Nilsson and H. Yamaguchi
Department of Pharmacology, University of Aarhus, Denmark.

Free cytosolic Ca2+ was measured with sub-micrometer-tip, double-barrelled, Ca(2+)-selective electrodes and fura 2 in rat mesenteric resistance arteries. The purpose was to establish intracellular free Ca2+ concentration ([Ca2+]i) values in resting and stimulated vessels. Isolated vessels were mounted for isometric force measurements. Measured with electrodes, mean [Ca2+]i was 115 and 708 nM under resting and norepinephrine-activated conditions, respectively. Fura 2 was calibrated intracellularly including determination of the intracellular dissociation constant (Kd) of the fura 2:Ca2+ complex. The intracellular Kd was 342 nM. With this value of Kd, fura 2 measurements of mean [Ca2+]i were 129 and 537 nM under resting and norepinephrine-activated conditions, respectively. The values measured with the two techniques were thus in good accordance.


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