AJP - Heart and Circulatory Physiology, Vol 265, Issue 6 1953-H1964, Copyright © 1993 by American Physiological Society

ARTICLES

Cytosolic calcium transients in myocytes isolated from rats with ischemic heart failure

J. M. Capasso, P. Li and P. Anversa
Department of Medicine, New York Medical College, Valhalla 10595.

Mechanical performance and cytosolic Ca2+ dynamics were characterized in myocytes isolated from left and right ventricles of rats with ischemic heart failure. Seven days after coronary artery narrowing (CAN) in rats filling pressures were elevated, whereas systolic pressures and ejection of blood were depressed. Left ventricular myocytes increased 18% in length and 19% in width, whereas right myocytes expanded longitudinally by 23% and transversely by 24%. Contractile behavior of myocytes displayed reductions in myocyte shortening and velocity of shortening, despite prolongation of time to peak shortening. Diastolic Ca2+ increased by 32 and 39% in left and right myocytes of CAN animals, whereas peak systolic Ca2+ in left ventricular myocytes was depressed (22%). Time to peak Ca2+ was prolonged by 68% in left myocytes. Moreover, time required for peak Ca2+ to return to diastolic levels was prolonged in left myocytes. Regression analysis revealed correlations between end-diastolic pressure and diastolic Ca2+ and peak developed pressure and systolic Ca2+. Thus ischemic heart failure finds its cellular basis in a depression in myocyte contractility that may in turn be due to alterations in cytosolic Ca2+ handling.

This article has been cited by other articles:

				G. H. Borchert, M. Giggey, F. Kolar, T. M. Wong, P. H. Backx, and P. V. Escriba 2-Hydroxyoleic acid affects cardiomyocyte [Ca2+]i transient and contractility in a region-dependent manner Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1948 - H1955. [Abstract] [Full Text] [PDF]

				J. P. Loennechen, U. Wisloff, G. Falck, and O. Ellingsen Effects of Cariporide and Losartan on Hypertrophy, Calcium Transients, Contractility, and Gene Expression in Congestive Heart Failure Circulation, March 19, 2002; 105(11): 1380 - 1386. [Abstract] [Full Text] [PDF]

				H. Yoshida and M. Karmazyn Na+/H+ exchange inhibition attenuates hypertrophy and heart failure in 1-wk postinfarction rat myocardium Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H300 - H304. [Abstract] [Full Text] [PDF]

				L. R. C. Dekker, H. Rademaker, J. T. Vermeulen, T. Opthof, R. Coronel, J. A. E. Spaan, and M. J. Janse Cellular Uncoupling During Ischemia in Hypertrophied and Failing Rabbit Ventricular Myocardium : Effects of Preconditioning Circulation, May 5, 1998; 97(17): 1724 - 1730. [Abstract] [Full Text] [PDF]

				G. Redaelli, A. Malhotra, B. Li, P. Li, E. H. Sonnenblick, P. A. Hofmann, and P. Anversa Effects of Constitutive Overexpression of Insulin-Like Growth Factor-1 on the Mechanical Characteristics and Molecular Properties of Ventricular Myocytes Circ. Res., March 23, 1998; 82(5): 594 - 603. [Abstract] [Full Text] [PDF]

				J. N. Tsoporis, A. Marks, H. J. Kahn, J. W. Butany, P. P. Liu, D. O'Hanlon, and T. G. Parker S100beta Inhibits alpha 1-Adrenergic Induction of the Hypertrophic Phenotype in Cardiac Myocytes J. Biol. Chem., December 12, 1997; 272(50): 31915 - 31921. [Abstract] [Full Text] [PDF]

				B. Li, Q. Li, X. Wang, K. P. Jana, G. Redaelli, J. Kajstura, and P. Anversa Coronary constriction impairs cardiac function and induces myocardial damage and ventricular remodeling in mice Am J Physiol Heart Circ Physiol, November 1, 1997; 273(5): H2508 - H2519. [Abstract] [Full Text] [PDF]

				H. S. Silverman, S.-k. Wei, M. C. P. Haigney, C. J. Ocampo, and M. D. Stern Myocyte Adaptation to Chronic Hypoxia and Development of Tolerance to Subsequent Acute Severe Hypoxia Circ. Res., May 19, 1997; 80(5): 699 - 707. [Abstract] [Full Text]

				G. Melillo, J. A.C. Lima, R. M. Judd, P. J. Goldschmidt-Clermont, and H. S. Silverman Intrinsic Myocyte Dysfunction and Tyrosine Kinase Pathway Activation Underlie the Impaired Wall Thickening of Adjacent Regions During Postinfarct Left Ventricular Remodeling Circulation, April 1, 1996; 93(7): 1447 - 1458. [Abstract] [Full Text]

				S. Boudina, M. N. Laclau, L. Tariosse, D. Daret, G. Gouverneur, S. Bonoron-Adele, V. A. Saks, and P. Dos Santos Alteration of mitochondrial function in a model of chronic ischemia in vivo in rat heart Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H821 - H831. [Abstract] [Full Text] [PDF]