| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Heart and Circulatory Physiology, Vol 266, Issue 3 1153-H1161, Copyright © 1994 by American Physiological Society
ARTICLES |
D. Diederich, J. Skopec, A. Diederich and F. X. Dai
Department of Medicine, University of Kansas Medical Center, Kansas City 66103.
Diabetes was induced in rats by an injection of streptozotocin (55 mg/kg). Endothelium-dependent relaxations in mesenteric resistance arteries (luminal diameter 210 +/- 20 microns) of control and diabetic rats were compared in myographs. Acetylcholine induced endothelium-dependent relaxations that were mediated by nitric oxide (EDNO). EDNO-mediated relaxations were impaired in diabetic arteries; concentrations of acetylcholine required to produce 50% relaxation (ED50) of activated arteries were 5 nM in control and 13.5 nM in arteries from diabetic rats studied after 6 wk (P < 0.05). The impairment in relaxation worsened with duration of the diabetes; ED50 for acetylcholine increased to 63 and 100 nM in diabetic arteries studied after 16 and 24 wk of diabetes, respectively. NG-nitro-L-arginine produced 5.5- and 16-fold decreases in sensitivity of control and diabetic arteries to acetylcholine. NG-nitro-L-arginine produced at least as much inhibition of acetylcholine relaxations in diabetic arteries, indicating that the impaired relaxation noted in diabetic arteries does not result from decreased production of EDNO. EDNO-mediated relaxations in diabetic arteries were impaired by increased production of endothelium-derived free radicals. Superoxide dismutase, a scavenger of superoxide anion, and dimethylthiourea, a scavenger of hydroxyl radicals, normalized EDNO-mediated relaxations in diabetic arteries. The ED50 values for acetylcholine were 13.5, 5.5, and 4 nM for untreated and SOD- and DMTU-treated diabetic arteries, respectively (P < 0.05 for treated vs. untreated arteries). Superoxide anion and hydroxyl radicals appear to block EDNO-mediated relaxation by inactivating EDNO.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  D. D. Lund, C. A. Gunnett, Y. Chu, R. M. Brooks, F. M. Faraci, and D. D. Heistad Gene transfer of extracellular superoxide dismutase improves relaxation of aorta after treatment with endotoxin Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H805 - H811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Fernandez-Real, G. Penarroja, A. Castro, F. Garcia-Bragado, A. Lopez-Bermejo, and W. Ricart Blood Letting in High-Ferritin Type 2 Diabetes: Effects on vascular reactivity Diabetes Care, December 1, 2002; 25(12): 2249 - 2255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sun and W. G. Mayhan Temporal effect of alcohol consumption on reactivity of pial arterioles: role of oxygen radicals Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H992 - H1001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hirai, H. Kawano, O. Hirashima, T. Motoyama, Y. Moriyama, T. Sakamoto, K. Kugiyama, H. Ogawa, K. Nakao, and H. Yasue Insulin resistance and endothelial dysfunction in smokers: effects of vitamin C Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H1172 - H1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zhao, X. Zhang, X. Xu, M. S. Wolin, and T. H. Hintze Depressed modulation of oxygen consumption by endogenous nitric oxide in cardiac muscle from diabetic dogs Am J Physiol Heart Circ Physiol, August 1, 2000; 279(2): H520 - H527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Lund, F. M. Faraci, F. J. Miller Jr, and D. D. Heistad Gene Transfer of Endothelial Nitric Oxide Synthase Improves Relaxation of Carotid Arteries From Diabetic Rabbits Circulation, March 7, 2000; 101(9): 1027 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Schoonmaker, R. W. Fallet, and P. K. Carmines Superoxide anion curbs nitric oxide modulation of afferent arteriolar ANG II responsiveness in diabetes mellitus Am J Physiol Renal Physiol, February 1, 2000; 278(2): F302 - F309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zhao, X. Zhang, C. J. Smith, X. Xu, M. Ochoa, D. Greenhouse, T. Vogel, C. Curran, and T. H. Hintze Reduced coronary NO production in conscious dogs after the development of alloxan-induced diabetes Am J Physiol Heart Circ Physiol, July 1, 1999; 277(1): H268 - H278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Kersten and D. C. Warltier Modulation of the adaptive response to myocardial ischemia by coexisting disease Am J Physiol Heart Circ Physiol, June 1, 1999; 276(6): H2268 - H2270. [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Mayhan and G. M. Sharpe Chronic exposure to nicotine alters endothelium-dependent arteriolar dilatation: effect of superoxide dismutase J Appl Physiol, April 1, 1999; 86(4): 1126 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. H. Thourani, W. S. Weintraub, B. Stein, S. S.P. Gebhart, J. M. Craver, E. L. Jones, and R. A. Guyton Influence of diabetes mellitus on early and late outcome after coronary artery bypass grafting Ann. Thorac. Surg., April 1, 1999; 67(4): 1045 - 1052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Szaleczky, J. Prechl, J. Fehér, and A. Somogyi Alterations in enzymatic antioxidant defence in diabetes mellitus - a rational approach Postgrad. Med. J., January 1, 1999; 75(879): 13 - 17. [Abstract] [Full Text]
|
|
|
|
|
|
W. G. Mayhan and G. M. Sharpe Superoxide dismutase restores endothelium-dependent arteriolar dilatation during acute infusion of nicotine J Appl Physiol, October 1, 1998; 85(4): 1292 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. G. Mayhan and K. P. Patel Treatment with dimethylthiourea prevents impaired dilatation of the basilar artery during diabetes mellitus Am J Physiol Heart Circ Physiol, June 1, 1998; 274(6): H1895 - H1901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Pieper Review of Alterations in Endothelial Nitric Oxide Production in Diabetes : Protective Role of Arginine on Endothelial Dysfunction Hypertension, May 1, 1998; 31(5): 1047 - 1060. [Full Text] [PDF]
|
|
|
|
 |
|
 F. M. FARACI and D. D. HEISTAD Regulation of the Cerebral Circulation: Role of Endothelium and Potassium Channels Physiol Rev, January 1, 1998; 78(1): 53 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hishikawa, B. S. Oemar, Z. Yang, and T. F. Luscher Pulsatile Stretch Stimulates Superoxide Production and Activates Nuclear Factor-{kappa}B in Human Coronary Smooth Muscle Circ. Res., November 19, 1997; 81(5): 797 - 803. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. M. Pieper and L. A. Dondlinger Plasma and Vascular Tissue Arginine Are Decreased in Diabetes: Acute Arginine Supplementation Restores Endothelium-Dependent Relaxation by Augmenting cGMP Production J. Pharmacol. Exp. Ther., November 1, 1997; 283(2): 684 - 691. [Abstract] [Full Text]
|
|
|
|
|
|
G. M. Pieper and W. Siebeneich Use of a Nitronyl Nitroxide to Discriminate the Contribution of Nitric Oxide Radical in Endothelium-Dependent Relaxation of Control and Diabetic Blood Vessels J. Pharmacol. Exp. Ther., October 1, 1997; 283(1): 138 - 147. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. J. Cipolla, J. M. Porter, G. Osol, and W. G. Mayhan High Glucose Concentrations Dilate Cerebral Arteries and Diminish Myogenic Tone Through an Endothelial Mechanism Stroke, February 1, 1997; 28(2): 405 - 411. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Erlich and T. Rosenthal Contribution of Nitric Oxide to the Beneficial Effects of Enalapril in the Fructose-Induced Hyperinsulinemic Rat Hypertension, November 1, 1996; 28(5): 754 - 757. [Abstract] [Full Text]
|
|
|
|
|
|
J. Angulo, C. F. Sanchez-Ferrer, C. Peiro, J. Marin, and L. Rodriguez-Manas Impairment of Endothelium-Dependent Relaxation by Increasing Percentages of Glycosylated Human Hemoglobin: Possible Mechanisms Involved Hypertension, October 1, 1996; 28(4): 583 - 592. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
