AJP - Heart and Circulatory Physiology, Vol 266, Issue 4 1439-H1445, Copyright © 1994 by American Physiological Society

ARTICLES

Assessment of cardiomyocyte DNA synthesis during hypertrophy in adult mice

M. H. Soonpaa and L. J. Field
Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis 46202-4800.

The ability of cardiomyocytes to synthesize DNA in response to experimentally induced cardiac hypertrophy was assessed in adult mice. Isoproterenol delivered by osmotic minipump implantation in adult C3Heb/FeJ mice resulted in a 46% increase in heart weight and a 19.3% increase in cardiomyocyte area. No DNA synthesis, as assessed by autoradiographic analysis of isolated cardiomyocytes, was observed in control or hypertrophic hearts. A survey of 15 independent inbred strains of mice revealed that ventricular cardiomyocyte nuclear number ranged from 3 to 13% mononucleate, suggesting that cardiomyocyte terminal differentiation is influenced directly or indirectly by genetic background. To determine whether the capacity for reactive DNA synthesis was also subject to genetic regulation, cardiac hypertrophy was induced in the strains of mice comprising the extremes of the nuclear number survey. These data indicate that adult mouse atrial and ventricular cardiomyocytes do not synthesize DNA in response to isoproterenol-induced cardiac hypertrophy.

This article has been cited by other articles:

				P. Ahuja, P. Sdek, and W. R. MacLellan Cardiac Myocyte Cell Cycle Control in Development, Disease, and Regeneration Physiol Rev, April 1, 2007; 87(2): 521 - 544. [Abstract] [Full Text] [PDF]

				K. L. March and B. H. Johnstone Cellular approaches to tissue repair in cardiovascular disease: the more we know, the more there is to learn Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H458 - H463. [Full Text] [PDF]

				M. Snir, I. Kehat, A. Gepstein, R. Coleman, J. Itskovitz-Eldor, E. Livne, and L. Gepstein Assessment of the ultrastructural and proliferative properties of human embryonic stem cell-derived cardiomyocytes Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2355 - H2363. [Abstract] [Full Text] [PDF]

				X.-S. Zhao, T. D. Gallardo, L. Lin, J. J. Schageman, and R. V. Shohet Transcriptional mapping and genomic analysis of the cardiac atria and ventricles Physiol Genomics, December 26, 2002; 12(1): 53 - 60. [Abstract] [Full Text] [PDF]

				B. Andree, A. Fleige, H.-H. Arnold, and T. Brand Mouse Pop1 Is Required for Muscle Regeneration in Adult Skeletal Muscle Mol. Cell. Biol., March 1, 2002; 22(5): 1504 - 1512. [Abstract] [Full Text] [PDF]

				H.-S. Liao, P. M. Kang, H. Nagashima, N. Yamasaki, A. Usheva, B. Ding, B. H. Lorell, and S. Izumo Cardiac-Specific Overexpression of Cyclin-Dependent Kinase 2 Increases Smaller Mononuclear Cardiomyocytes Circ. Res., March 2, 2001; 88(4): 443 - 450. [Abstract] [Full Text] [PDF]

				K. B. S. Pasumarthi, H. Nakajima, H. O. Nakajima, S. Jing, and L. J. Field Enhanced Cardiomyocyte DNA Synthesis During Myocardial Hypertrophy in Mice Expressing a Modified TSC2 Transgene Circ. Res., May 26, 2000; 86(10): 1069 - 1077. [Abstract] [Full Text] [PDF]

				N. Saadane, L. Alpert, and L. E. Chalifour Altered molecular response to adrenoreceptor-induced cardiac hypertrophy in Egr-1-deficient mice Am J Physiol Heart Circ Physiol, March 1, 2000; 278(3): H796 - H805. [Abstract] [Full Text] [PDF]

				P. Anversa and J. Kajstura Ventricular Myocytes Are Not Terminally Differentiated in the Adult Mammalian Heart Circ. Res., July 13, 1998; 83(1): 1 - 14. [Full Text] [PDF]

				M. H. Soonpaa and L. J. Field Survey of Studies Examining Mammalian Cardiomyocyte DNA Synthesis Circ. Res., July 13, 1998; 83(1): 15 - 26. [Full Text] [PDF]

				K. K. Kim, A. I. Daud, S. C. Wong, L. Pajak, S.-C. Tsai, H. Wang, W. J. Henzel, and L. J. Field Mouse RAD50 Has Limited Epitopic Homology to p53 and Is Expressed in the Adult Myocardium J. Biol. Chem., November 15, 1996; 271(46): 29255 - 29264. [Abstract] [Full Text] [PDF]

				P. L. Kozlovskis, M. J.D. Smets, W. L. Strauss, and R. J. Myerburg DNA Synthesis in Adult Feline Ventricular Myocytes : Comparison of Hypoxic and Normoxic States Circ. Res., February 1, 1996; 78(2): 289 - 301. [Abstract] [Full Text]

				C. J. Friddle, T. Koga, E. M. Rubin, and J. Bristow Expression profiling reveals distinct sets of genes altered during induction and regression of cardiac hypertrophy PNAS, June 6, 2000; 97(12): 6745 - 6750. [Abstract] [Full Text] [PDF]

				A. P. Meeson, N. Radford, J. M. Shelton, P. P. A. Mammen, J. M. DiMaio, K. Hutcheson, Y. Kong, J. Elterman, R. S. Williams, and D. J. Garry Adaptive Mechanisms That Preserve Cardiac Function in Mice Without Myoglobin Circ. Res., April 13, 2001; 88(7): 713 - 720. [Abstract] [Full Text] [PDF]

				G. Xiao, S. Mao, G. Baumgarten, J. Serrano, M. C. Jordan, K. P. Roos, M. C. Fishbein, and W. R. MacLellan Inducible Activation of c-Myc in Adult Myocardium In Vivo Provokes Cardiac Myocyte Hypertrophy and Reactivation of DNA Synthesis Circ. Res., December 7, 2001; 89(12): 1122 - 1129. [Abstract] [Full Text] [PDF]