| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Heart and Circulatory Physiology, Vol 268, Issue 1 100-H111, Copyright © 1995 by American Physiological Society
ARTICLES |
A. Meissner and J. P. Morgan
Charles A. Dana Research Institute, Boston, Massachusetts.
Isolated adult rat hearts in an isovolumic nonworking Langendorff preparation were loaded with the Ca2+ indicator aequorin to investigate the effects of ischemic reperfusion on free intracellular Ca2+ concentration ([Ca2+]i) homeostasis and left ventricular (LV) contractile function. In three groups (each n = 8) that underwent 10, 20, and 30 min of ischemia, recovery of developed pressure amounted to, respectively, 63% [77 +/- 3 (SE) mmHg], 48% (56 +/- 4 mmHg), and 34% (43 +/- 4 mmHg) of preischemic control (122 +/- 5 mmHg) after 60 min of reperfusion. Diastolic pressure remained elevated at 40 +/- 4, 55 +/- 3, and 65 +/- 6 mmHg, respectively (preischemic control, 12 mmHg). During early reperfusion (0-20 min), the light transient demonstrated a prolonged time to 90% decline from peak light (t90L), which was paralleled by a delayed relaxation on the LV pressure tracing in the 10- and 20-min ischemia groups. After 60 min of reperfusion, the prolongation of t90L persisted in all groups (10-min ischemia, 89 +/- 2 ms; 20 min, 95 +/- 3 ms; 30 min, 96 +/- 2 ms; control, 82 +/- 2 ms; P < 0.05). In contrast, the LV pressure tracing was abbreviated beyond the preischemic control, indicating altered myofibrillar Ca2+ responsiveness. Diastolic [Ca2+]i was elevated after 60 min of reperfusion (10-min ischemia, 0.40 +/- 0.06 microM; 20 min, 0.48 +/- 0.04 microM; 30 min, 0.51 +/- 0.06 microM; control, 0.32 +/- 0.01 microM) and had a significant positive correlation with LV diastolic pressure (r = 0.79; P < 0.001). A positive correlation was also found for the amplitude of the Ca2+ transient and LV developed pressure (r = 0.53; P < 0.05). These findings suggest that postischemic contractile dysfunction is related to altered Ca2+ modulation with impaired [Ca2+]i homeostasis following moderate to severe reperfusion injury in the rat.
This article has been cited by other articles:
|
|
 |
|
  H. K. Saini, V. Elimban, and N. S. Dhalla Attenuation of extracellular ATP response in cardiomyocytes isolated from hearts subjected to ischemia-reperfusion Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H614 - H623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. K. Saini and N. S. Dhalla Defective calcium handling in cardiomyocytes isolated from hearts subjected to ischemia-reperfusion Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2260 - H2270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Eerbeek, E. G. Mik, C. J. Zuurbier, M. v. t Loo, C. Donkersloot, and C. Ince Ratiometric intracellular calcium imaging in the isolated beating rat heart using indo-1 fluorescence J Appl Physiol, December 1, 2004; 97(6): 2042 - 2050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Min, H. Liao, J.-F. Wang, M. F. Sullivan, T. Ito, and J. P. Morgan Genistein Attenuates Postischemic Depressed Myocardial Function by Increasing Myofilament Ca2+ Sensitivity in Rat Myocardium Experimental Biology and Medicine, September 1, 2002; 227(8): 632 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Nicolosi, G. West, J. G. Markley, B. Logan, and G. N. Olinger Gadolinium attenuates regional stunning in the canine heart in vivo J. Thorac. Cardiovasc. Surg., July 1, 2002; 124(1): 57 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Nicolosi, C. S. Kwok, S. J. Contney, G. N. Olinger, and Z. J. Bosnjak Gadolinium prevents stretch-mediated contractile dysfunction in isolated papillary muscles Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H1122 - H1128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Hampton, J.-F. Wang, J. DeAngelis, I. Amende, K. D. Philipson, and J. P. Morgan Enhanced gene expression of Na+/Ca2+ exchanger attenuates ischemic and hypoxic contractile dysfunction Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H2846 - H2854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. R. Eberli, H. Stromer, M. A. Ferrell, N. Varma, J. P. Morgan, S. Neubauer, and C. S. Apstein Lack of Direct Role for Calcium in Ischemic Diastolic Dysfunction in Isolated Hearts Circulation, November 21, 2000; 102(21): 2643 - 2649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bellomo, J. P. Headrick, G. U. Silins, C. A. Paterson, P. S. Thomas, M. Gartside, A. Mould, M. M. Cahill, I. D. Tonks, S. M. Grimmond, et al. Mice Lacking the Vascular Endothelial Growth Factor-B Gene (Vegfb) Have Smaller Hearts, Dysfunctional Coronary Vasculature, and Impaired Recovery From Cardiac Ischemia Circ. Res., February 4, 2000; 86 (2): e29 - e35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. O'Donnell, L. T. White, and E. D. Lewandowski Mitochondrial transporter responsiveness and metabolic flux homeostasis in postischemic hearts Am J Physiol Heart Circ Physiol, September 1, 1999; 277(3): H866 - H873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Kiss and S. J. Korn Modulation of N-Type Ca2+ Channels by Intracellular pH in Chick Sensory Neurons J Neurophysiol, April 1, 1999; 81(4): 1839 - 1847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cittadini, Y. Ishiguro, H. Stromer, M. Spindler, A. C. Moses, R. Clark, P. S. Douglas, J. S. Ingwall, and J. P. Morgan Insulin-like Growth Factor-1 but Not Growth Hormone Augments Mammalian Myocardial Contractility by Sensitizing the Myofilament to Ca2+ Through a Wortmannin-Sensitive Pathway : Studies in Rat and Ferret Isolated Muscles Circ. Res., June 13, 1998; 83(1): 50 - 59. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Hampton, I. Amende, K. E. Travers, and J. P. Morgan Intracellular calcium dynamics in mouse model of myocardial stunning Am J Physiol Heart Circ Physiol, May 1, 1998; 274(5): H1821 - H1827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. P. Matherne, J. Linden, A. M. Byford, N. S. Gauthier, and J. P. Headrick Transgenic A1 adenosine receptor overexpression increases myocardial resistance to ischemia PNAS, June 10, 1997; 94(12): 6541 - 6546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Stromer, A. Cittadini, P. S. Douglas, and J. P. Morgan Exogenously Administered Growth Hormone and Insulin-like Growth Factor-I Alter Intracellular Ca2+ Handling and Enhance Cardiac Performance: In Vitro Evaluation in the Isolated Isovolumic Buffer-Perfused Rat Heart Circ. Res., August 1, 1996; 79(2): 227 - 236. [Abstract] [Full Text]
|
|
|
|
|
|
A. Cittadini, H. Stromer, S. E. Katz, R. Clark, A. C. Moses, J. P. Morgan, and P. S. Douglas Differential Cardiac Effects of Growth Hormone and Insulin-like Growth Factor1 in the Rat : A Combined In Vivo and In Vitro Evaluation Circulation, February 15, 1996; 93(4): 800 - 809. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
