| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Heart and Circulatory Physiology, Vol 268, Issue 1 48-H55, Copyright © 1995 by American Physiological Society
ARTICLES |
J. E. Schnitzer, J. Allard and P. Oh
Department of Pathology, Harvard Medical School, Beth Israel Hospital, Boston, Massachusetts 02215.
Various vesicular carriers transport select molecular cargo between intracellular compartments utilizing a budding mechanism with docking and fusion of individual vesicles with their target membranes. This fusion requires key intracellular component(s) that are sensitive to alkylation with N-ethylmaleimide (NEM). In endothelium, caveolae may mediate endocytosis and transcytosis of select macromolecules. If caveolae utilize a mechanism similar to other vesicular carriers, then their transport should also be sensitive to NEM. The following tracers were chosen based on their pathway specificity: 1) albumin-gold complexes (A-Au) that bind gp30 and gp18 for endocytosis by caveolae, 2) native albumin that binds albondin and is transported by caveolae, 3) ferritin as a fluid-phase probe transcytosed by caveolae, and 4) inulin as a paracellular probe. In culture, NEM significantly inhibited A-Au uptake and delivery to endosomes but not endothelial cell surface binding. In rat lung, NEM reduced capillary permeability to albumin and the tissue uptake of ferritin and A-Au, but not inulin, indicating inhibition of caveolae-mediated but not paracellular transport. Neutral but not charged alkylating agents inhibited A-Au uptake, consistent with their relative abilities to cross membranes and modify intracellular factors. Like other vesicular pathways, endothelial caveolae transport their select ligands utilizing a NEM-sensitive mechanism, apparently requiring vesicle-membrane fusion.
This article has been cited by other articles:
|
|
 |
|
  S. A. Predescu, D. N. Predescu, and A. B. Malik Molecular determinants of endothelial transcytosis and their role in endothelial permeability Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L823 - L842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Cancel, A. Fitting, and J. M. Tarbell In vitro study of LDL transport under pressurized (convective) conditions Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H126 - H132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-I. Rosengren, A. Rippe, C. Rippe, D. Venturoli, K. Sward, and B. Rippe Transvascular protein transport in mice lacking endothelial caveolae Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1371 - H1377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Bito, S. Hino, A. Baba, M. Tanaka, H. Watabe, and H. Kawabata Degradation of oxidative stress-induced denatured albumin in rat liver endothelial cells Am J Physiol Cell Physiol, September 1, 2005; 289(3): C531 - C542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Parker and M. I. Townsley Evaluation of lung injury in rats and mice Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L231 - L246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. TUMA and A. L. HUBBARD Transcytosis: Crossing Cellular Barriers Physiol Rev, July 1, 2003; 83(3): 871 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ring, J. Pohl, A. Volkl, and W. Stremmel Evidence for vesicles that mediate long-chain fatty acid uptake by human microvascular endothelial cells J. Lipid Res., December 1, 2002; 43(12): 2095 - 2104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Razani, S. E. Woodman, and M. P. Lisanti Caveolae: From Cell Biology to Animal Physiology Pharmacol. Rev., September 1, 2002; 54(3): 431 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Knight, P. Bodin, W. C. De Groat, and G. Burnstock ATP is released from guinea pig ureter epithelium on distension Am J Physiol Renal Physiol, February 1, 2002; 282(2): F281 - F288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Carlsson, B.-I. Rosengren, and B. Rippe Transcytosis inhibitor N-ethylmaleimide increases microvascular permeability in rat muscle Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1728 - H1733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Dvorak and D. Feng The Vesiculo-Vacuolar Organelle (VVO): A New Endothelial Cell Permeability Organelle J. Histochem. Cytochem., April 1, 2001; 49(4): 419 - 432. [Abstract] [Full Text]
|
|
|
|
|
|
D. Feng, R. Flaumenhaft, C. Bandeira–Melo, P. F. Weller, and A. M. Dvorak Ultrastructural Localization of Vesicle-associated Membrane Protein(s) to Specialized Membrane Structures in Human Pericytes, Vascular Smooth Muscle Cells, Endothelial Cells, Neutrophils, and Eosinophils J. Histochem. Cytochem., March 1, 2001; 49(3): 293 - 304. [Abstract] [Full Text]
|
|
|
|
|
|
B. Rippe and A. Taylor NEM and filipin increase albumin transport in lung microvessels Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H34 - H41. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Rowe and D. J. Bobilya Albumin Facilitates Zinc Acquisition by Endothelial Cells Experimental Biology and Medicine, July 1, 2000; 224(3): 178 - 186. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Frolov, A. Petrescu, B. P. Atshaves, P. T. C. So, E. Gratton, G. Serrero, and F. Schroeder High Density Lipoprotein-mediated Cholesterol Uptake and Targeting to Lipid Droplets in Intact L-cell Fibroblasts. A SINGLE- AND MULTIPHOTON FLUORESCENCE APPROACH J. Biol. Chem., April 21, 2000; 275(17): 12769 - 12780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Fenart, A. Casanova, B. Dehouck, C. Duhem, S. Slupek, R. Cecchelli, and D. Betbeder Evaluation of Effect of Charge and Lipid Coating on Ability of 60-nm Nanoparticles to Cross an In Vitro Model of the Blood-Brain Barrier J. Pharmacol. Exp. Ther., December 1, 1999; 291(3): 1017 - 1022. [Abstract] [Full Text]
|
|
|
|
|
|
D. P. McIntosh and J. E. Schnitzer Caveolae require intact VAMP for targeted transport in vascular endothelium Am J Physiol Heart Circ Physiol, December 1, 1999; 277(6): H2222 - H2232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Michel and F. E. Curry Microvascular Permeability Physiol Rev, July 1, 1999; 79(3): 703 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Vasile, Qu-Hong, H. F. Dvorak, and A. M. Dvorak Caveolae and Vesiculo–Vacuolar Organelles in Bovine Capillary Endothelial Cells Cultured with VPF/VEGF on Floating Matrigel–collagen Gels J. Histochem. Cytochem., February 1, 1999; 47(2): 159 - 168. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Predescu, S. Predescu, T. McQuistan, and G. E. Palade Transcytosis of alpha 1-acidic glycoprotein in the continuous microvascular endothelium PNAS, May 26, 1998; 95(11): 6175 - 6180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Cameron, J. W. Ruffin, R. Bollag, H. Rasmussen, and R. S. Cameron Identification of Caveolin and Caveolin-Related Proteins in the Brain J. Neurosci., December 15, 1997; 17(24): 9520 - 9535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu, P. Oh, T. Horner, R. A. Rogers, and J. E. Schnitzer Organized Endothelial Cell Surface Signal Transduction in Caveolae Distinct from Glycosylphosphatidylinositol-anchored Protein Microdomains J. Biol. Chem., March 14, 1997; 272(11): 7211 - 7222. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
