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AJP - Heart and Circulatory Physiology, Vol 269, Issue 5 1528-H1537, Copyright © 1995 by American Physiological Society
ARTICLES |
A. L. Baldwin and G. Thurston
Department of Physiology, College of Medicine, University of Arizona, Tucson 85724, USA.
In this study the time course of development and recovery of histamine-induced venular leaks was followed in conjunction with rearrangement of endothelial actin fibers. The microvasculature of a single mesenteric window of anesthetized Sprague-Dawley rats was perfused with buffered saline, with or without 10(-4) M histamine, for 3-30 min. Fluorescein isothiocyanate (FITC)-albumin was added for the last 3 min. The microvasculature was perfusion fixed, stained with rhodamine phalloidin (for filamentous actin), and viewed using confocal microscopy. The number and relative size of FITC-albumin leaks per venule length were measured. After 3 min of histamine application focal leaks appeared in some of the venules. Most focal leaks were accompanied by local breaks in the endothelial peripheral actin rim. Larger leaks were also present, accompanied by greater disruption of the venular endothelial peripheral actin rim, diffuse F-actin staining, and adherent platelets and leukocytes. Few central actin fibers were visible even in endothelial cells associated with large leaks. After 10-15 min of histamine exposure, larger leaks were more abundant but with fewer adherent cells. Central actin fibers in endothelial cells increased in number, peaking after 20 min of histamine, while the diffuse actin staining declined. Leak area per micrometer of venule peaked at 10-15 min, but the numbers of leaks per micrometer did not vary significantly from 3 to 30 min. These data suggest that the central fibers are not involved with the phase of increasing permeability, but they may play a role in the structural and functional recovery of endothelial cells perturbed by histamine.
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J. W. Breslin and S. Y. Yuan Involvement of RhoA and Rho kinase in neutrophil-stimulated endothelial hyperpermeability Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H1057 - H1062. [Abstract] [Full Text] [PDF]
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A. L. Baldwin, E. B. Wiley, A. G. Summers, and A. I. Alayash Sodium selenite reduces hemoglobin-induced venular leakage in the rat mesentery Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H81 - H91. [Abstract] [Full Text] [PDF]
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J. L. Underwood, C. G. Murphy, J. Chen, L. Franse-Carman, I. Wood, D. L. Epstein, and J. A. Alvarado Glucocorticoids regulate transendothelial fluid flow resistance and formation of intercellular junctions Am J Physiol Cell Physiol, August 1, 1999; 277(2): C330 - C342. [Abstract] [Full Text] [PDF]
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J. E. Valeski and A. L. Baldwin Effect of early transient adherent leukocytes on venular permeability and endothelial actin cytoskeleton Am J Physiol Heart Circ Physiol, August 1, 1999; 277(2): H569 - H575. [Abstract] [Full Text] [PDF]
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A. L. Baldwin Modified hemoglobins produce venular interendothelial gaps and albumin leakage in the rat mesentery Am J Physiol Heart Circ Physiol, August 1, 1999; 277(2): H650 - H659. [Abstract] [Full Text] [PDF]
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R. K. Wong, A. L. Baldwin, and R. L. Heimark Cadherin-5 redistribution at sites of TNF-alpha and IFN-gamma -induced permeability in mesenteric venules Am J Physiol Heart Circ Physiol, February 1, 1999; 276(2): H736 - H748. [Abstract] [Full Text] [PDF]
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Y. Yuan, F. Y. Meng, Q. Huang, J. Hawker, and H. Mac Wu Tyrosine phosphorylation of paxillin/pp125FAK and microvascular endothelial barrier function Am J Physiol Heart Circ Physiol, July 1, 1998; 275(1): H84 - H93. [Abstract] [Full Text] [PDF]
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A. L. Baldwin, G. Thurston, and H. Al Naemi Inhibition of nitric oxide synthesis increases venular permeability and alters endothelial actin cytoskeleton Am J Physiol Heart Circ Physiol, May 1, 1998; 274(5): H1776 - H1784. [Abstract] [Full Text] [PDF]
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