AJP - Heart and Circulatory Physiology, Vol 269, Issue 5 1672-H1678, Copyright © 1995 by American Physiological Society

ARTICLES

Role of nitric oxide in adenosine receptor-mediated relaxation of porcine coronary artery

W. Abebe, T. Hussain, H. Olanrewaju and S. J. Mustafa
Department of Pharmacology, School of Medicine, East Carolina University, Greenville, North Carolina 27858-4354, USA.

In the present study, using porcine coronary artery rings in vitro, we examined the role of the nitric oxide (NO) pathway in endothelium-dependent vasorelaxant effects of the 5'-uronamide adenosine agonists, 5'-(N-ethylcarboxamido)adenosine (NECA) and 2-[p-(2-carboxyethyl)]phenylethyl-amino-5'-N-ethylcarboxamidoadenosine (CGS-21680) as opposed to the endothelium-independent actions of the C2- and N6-substituted analogues, 2-chloroadenosine (CAD) and N6-cyclopentyladenosine (CPA). The NO synthase inhibitor, NG-monomethyl-L-arginine (L-NMMA, 30 microM), and the NO-destroying agent, 6-anilino-5,8-quinolinedione (LY-83583, 10 microM), attenuated the relaxations of endothelium-intact but not -denuded rings to NECA and CGS-21680. The effect of L-NMMA on NECA-induced relaxation was reversed by L-arginine (100 microM), a substrate for NO synthesis. In the endothelium-intact tissues, both NECA and CGS-21680 elicited enhanced production of nitrite, a stable metabolite of NO. This was also attenuated by L-NMMA or endothelium removal. Furthermore, NECA (10 microM) induced augmentation of guanosine 3',5'-cyclic monophosphate (cGMP) production in the intact arteries, which was also inhibited by L-NMMA, LY-83583, or endothelium removal. In contrast, vasorelaxant responses generated by CAD and CPA were not altered by either L-NMMA or LY-83583. Both agents (10 microM) were also unable to alter nitrite and/or guanosine 3',5'-cyclic monophosphate (cGMP) levels of the coronary artery.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				M. A. Nayeem, S. M. Poloyac, J. R. Falck, D. C. Zeldin, C. Ledent, D. S. Ponnoth, H. R. Ansari, and S. J. Mustafa Role of CYP epoxygenases in A2AAR-mediated relaxation using A2AAR-null and wild-type mice Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H2068 - H2078. [Abstract] [Full Text] [PDF]

				H. R. Ansari, A. Nadeem, M. A. H. Talukder, S. Sakhalkar, and S. J. Mustafa Evidence for the involvement of nitric oxide in A2B receptor-mediated vasorelaxation of mouse aorta Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H719 - H725. [Abstract] [Full Text] [PDF]

				M.-h. Kim and N. R. Harris Leukocyte adherence inhibits adenosine-dependent venular control of arteriolar diameter and nitric oxide Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H724 - H731. [Abstract] [Full Text] [PDF]

				P. B. Hansen and J. Schnermann Vasoconstrictor and vasodilator effects of adenosine in the kidney Am J Physiol Renal Physiol, October 1, 2003; 285(4): F590 - F599. [Abstract] [Full Text] [PDF]

				R. Rubio and G. Ceballos Sole activation of three luminal adenosine receptor subtypes in different parts of coronary vasculature Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H204 - H214. [Abstract] [Full Text] [PDF]

				A. W. WYATT, J. R. STEINERT, C. P. D. WHEELER-JONES, A. J. MORGAN, D. SUGDEN, J. D. PEARSON, L. SOBREVIA, and G. E. MANN Early activation of the p42/p44MAPK pathway mediates adenosine-induced nitric oxide production in human endothelial cells: a novel calcium-insensitive mechanism FASEB J, October 1, 2002; 16(12): 1584 - 1594. [Abstract] [Full Text] [PDF]

				T. J. Bivalacqua, H. C. Champion, D. G. Lambert, and P. J. Kadowitz Vasodilator responses to adenosine and hyperemia are mediated by A1 and A2 receptors in the cat vascular bed Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1696 - R1709. [Abstract] [Full Text] [PDF]

				R. D. Lasley, M. S. Jahania, and R. M. Mentzer Jr. Beneficial effects of adenosine A2a agonist CGS-21680 in infarcted and stunned porcine myocardium Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1660 - H1666. [Abstract] [Full Text] [PDF]

				E. P. Silldorff and T. L. Pallone Adenosine signaling in outer medullary descending vasa recta Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2001; 280(3): R854 - R861. [Abstract] [Full Text] [PDF]

				L. J. Rubin, L. R. Johnson, J. R. Dodam, A. K. Dhalla, L. Magliola, M. H. Laughlin, and A. W. Jones Selective transport of adenosine into porcine coronary smooth muscle Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H1397 - H1410. [Abstract] [Full Text] [PDF]

				H. A. Olanrewaju, W. Qin, I. Feoktistov, J.-L. Scemama, and S. J. Mustafa Adenosine A2A and A2B receptors in cultured human and porcine coronary artery endothelial cells Am J Physiol Heart Circ Physiol, August 1, 2000; 279(2): H650 - H656. [Abstract] [Full Text] [PDF]

				T. W. Hein, L. Belardinelli, and L. Kuo Adenosine A2A Receptors Mediate Coronary Microvascular Dilation to Adenosine: Role of Nitric Oxide and ATP-Sensitive Potassium Channels J. Pharmacol. Exp. Ther., November 1, 1999; 291(2): 655 - 664. [Abstract] [Full Text]

				T. W. Hein and L. Kuo cAMP-Independent Dilation of Coronary Arterioles to Adenosine : Role of Nitric Oxide, G Proteins, and KATP Channels Circ. Res., October 1, 1999; 85(7): 634 - 642. [Abstract] [Full Text] [PDF]

				P. B. Anning, B. D. Prendergast, P. A. MacCarthy, A. M. Shah, D. C. Buss, and M. J. Lewis ATP is involved in myocardial and vascular effects of exogenous bradykinin in ejecting guinea pig heart Am J Physiol Heart Circ Physiol, August 1, 1999; 277(2): H818 - H825. [Abstract] [Full Text] [PDF]

				G. S. Huggins, R. C. Pasternak, N. M. Alpert, A. J. Fischman, and H. Gewirtz Effects of Short-Term Treatment of Hyperlipidemia on Coronary Vasodilator Function and Myocardial Perfusion in Regions Having Substantial Impairment of Baseline Dilator Reverse Circulation, September 29, 1998; 98(13): 1291 - 1296. [Abstract] [Full Text] [PDF]

				W. Abebe and S. J. Mustafa Effect of Low Density Lipoprotein on Adenosine Receptor-Mediated Coronary Vasorelaxation in Vitro J. Pharmacol. Exp. Ther., August 1, 1997; 282(2): 851 - 857. [Abstract] [Full Text]

				L. Belardinelli, J. C. Shryock, J. Ruble, A. Monopoli, S. Dionisotti, E. Ongini, D. M. Dennis, and S. P. Baker Binding of the Novel Nonxanthine A2A Adenosine Receptor Antagonist [3H]SCH58261 to Coronary Artery Membranes Circ. Res., December 1, 1996; 79(6): 1153 - 1160. [Abstract] [Full Text]