AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 270: H259-H266, 1996;
0363-6135/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, W.
Right arrow Articles by Fliegel, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, W.
Right arrow Articles by Fliegel, L.

AJP - Heart and Circulatory Physiology, Vol 270, Issue 1 259-H266, Copyright © 1996 by American Physiological Society

ARTICLES

Regulation of NHE-1 promoter in mammalian myocardium

W. Yang, J. R. Dyck, H. Wang and L. Fliegel
Department of Biochemistry, Faculty of Medicine, University of Alberta, Edmonton, Canada.

The Na+/H exchanger (NHE-1) is an integral membrane protein responsible for intracellular pH regulation in the myocardium and other tissues. The NHE-1 isoform is universally distributed in mammalian cells. We examined regulation of a 1.1-kb fragment of the NHE-1 promoter in neonatal rat cardiomyocytes. Deletion of most of the promoter up to an AP-2 site reduced activity 75%. Further deletion of the promoter or mutation of the AP-2 site reduced or eliminated activity almost completely. Gel mobility shift assay showed that purified AP-2 protein or AP-2-like protein from nuclear extracts of isolated myocytes can bind to DNA of the NHE-1 protein. External acidosis did not cause increased transcription from the promoter. Removal of serum from the medium reduced activity of the NHE-1 promoter. The elements responsible for activation of the promoter by serum were contained within both the 1.1-kb and AP-2-containing region. The results show that the cis-acting putative AP-2 site and the presence of serum are important in NHE-1 expression, whereas external acidosis had no direct effect on the promoter.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Malakooti, R. Sandoval, V. C. Memark, P. K. Dudeja, and K. Ramaswamy
Zinc finger transcription factor Egr-1 is involved in stimulation of NHE2 gene expression by phorbol 12-myristate 13-acetate
Am J Physiol Gastrointest Liver Physiol, October 1, 2005; 289(4): G653 - G663.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Malakooti, V. C. Memark, P. K. Dudeja, and K. Ramaswamy
Molecular cloning and functional analysis of the human Na+/H+ exchanger NHE3 promoter
Am J Physiol Gastrointest Liver Physiol, March 1, 2002; 282(3): G491 - G500.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Karmazyn, X. T. Gan, R. A Humphreys, H. Yoshida, and K. Kusumoto
The Myocardial Na+-H+ Exchange : Structure, Regulation, and Its Role in Heart Disease
Circ. Res., October 29, 1999; 85(9): 777 - 786.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
X. T. Gan, S. Chakrabarti, and M. Karmazyn
Modulation of Na+/H+ exchange isoform 1 mRNA expression in isolated rat hearts
Am J Physiol Heart Circ Physiol, September 1, 1999; 277(3): H993 - H998.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. Besson, F. Fernandez-Rachubinski, W. Yang, and L. Fliegel
Regulation of Na+/H+ exchanger gene expression: mitogenic stimulation increases NHE1 promoter activity
Am J Physiol Cell Physiol, March 1, 1998; 274(3): C831 - C839.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online