AJP - Heart Calcium Transients and Cell-Sarcomere
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 270: H1462-H1468, 1996;
0363-6135/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wung, B. S.
Right arrow Articles by Wang, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wung, B. S.
Right arrow Articles by Wang, D. L.

AJP - Heart and Circulatory Physiology, Vol 270, Issue 4 1462-H1468, Copyright © 1996 by American Physiological Society

ARTICLES

Cyclical strain increases monocyte chemotactic protein-1 secretion in human endothelial cells

B. S. Wung, J. J. Cheng, Y. J. Chao, J. Lin, Y. J. Shyy and D. L. Wang
Cardiovascular Division, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.

The effects of mechanical strain on monocyte chemotactic protein-1 (MCP-1) secretion were examined on human endothelial cells (ECs) grown on a flexible membrane base. MCP-1 release into culture medium from strained ECs was demonstrated to be time and strain dose dependent. Northern blot analysis demonstrated a mainly serum-independent 1.8-fold induction of MCP-1 mRNA levels in ECs strained at 15 kPa compared with unstrained controls. ECs treated with actinomycin D abolished this strain-induced expression. Strained ECs at the periphery of wells showed higher MCP-1 gene expression than ECs at the center. Pretreatment of ECs with either cytochalasin D or phalloidin did not abolish strain-induced gene expression. ECs pretreated with stretch-activated ion channel blocker gadolinium or with ryanodine to deplete intracellular stored Ca2+ strongly inhibited the strain-induced MCP-1 levels. We conclude that 1) cyclical strain can modulate the secretion of MCP-1 in a dose-dependent manner, 2) strain-induced MCP-1 production is mediated by increasing MCP-1 mRNA levels via transcription, 3) cytoskeletal rearrangement is not essential for this strain-induced MCP-1 expression, and 4) both Ca2+ influx via stretch-activated ion channels and intracellular Ca2+ release contribute to the strain-induced effect. Such strain-induced MCP-1 secretion might contribute to the trapping of monocytes in the subendothelial space to initiate atherogenesis.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
E. L. Schiffrin and R. M. Touyz
From bedside to bench to bedside: role of renin-angiotensin-aldosterone system in remodeling of resistance arteries in hypertension
Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H435 - H446.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
H. KIMURA, O. OKADA, N. TANABE, Y. TANAKA, M. TERAI, Y. TAKIGUCHI, M. MASUDA, N. NAKAJIMA, K. HIROSHIMA, H. INADERA, et al.
Plasma Monocyte Chemoattractant Protein-1 and Pulmonary Vascular Resistance in Chronic Thromboembolic Pulmonary Hypertension
Am. J. Respir. Crit. Care Med., July 15, 2001; 164(2): 319 - 324.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
I.-W. Park, J.-F. Wang, and J. E. Groopman
HIV-1 Tat promotes monocyte chemoattractant protein-1 secretion followed by transmigration of monocytes
Blood, January 15, 2001; 97(2): 352 - 358.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Pugin, I. Dunn, P. Jolliet, D. Tassaux, J.-L. Magnenat, L. P. Nicod, and J.-C. Chevrolet
Activation of human macrophages by mechanical ventilation in vitro
Am J Physiol Lung Cell Mol Physiol, December 1, 1998; 275(6): L1040 - L1050.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Okada, A. Matsumori, K. Ono, Y. Furukawa, T. Shioi, A. Iwasaki, K. Matsushima, and S. Sasayama
Cyclic Stretch Upregulates Production of Interleukin-8 and Monocyte Chemotactic and Activating Factor/Monocyte Chemoattractant Protein-1 in Human Endothelial Cells
Arterioscler. Thromb. Vasc. Biol., June 1, 1998; 18(6): 894 - 901.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
B. S. Wung, J. J. Cheng, H. J. Hsieh, Y. J. Shyy, and D. L. Wang
Cyclic Strain–Induced Monocyte Chemotactic Protein-1 Gene Expression in Endothelial Cells Involves Reactive Oxygen Species Activation of Activator Protein 1
Circ. Res., July 19, 1997; 81(1): 1 - 7.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
W. D. Ito, M. Arras, B. Winkler, D. Scholz, J. Schaper, and W. Schaper
Monocyte Chemotactic Protein-1 Increases Collateral and Peripheral Conductance After Femoral Artery Occlusion
Circ. Res., June 19, 1997; 80(6): 829 - 837.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. DREYFUSS and G. SAUMON
Ventilator-induced Lung Injury . Lessons from Experimental Studies
Am. J. Respir. Crit. Care Med., January 1, 1997; 157(1): 294 - 323.
[Full Text]

Home page
 J. Biol. Chem.Home page
J.-J. Cheng, B.-S. Wung, Y.-J. Chao, and D. L. Wang
Sequential Activation of Protein Kinase C (PKC)-alpha and PKC-epsilon Contributes to Sustained Raf/ERK1/2 Activation in Endothelial Cells under Mechanical Strain
J. Biol. Chem., August 10, 2001; 276(33): 31368 - 31375.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online