AJP - Heart and Circulatory Physiology, Vol 270, Issue 5 1549-H1556, Copyright © 1996 by American Physiological Society

ARTICLES

Transport of tracer albumin from peritoneum to plasma: role of diaphragmatic, visceral, and parietal lymphatics

E. R. Zakaria, O. Simonsen, A. Rippe and B. Rippe
Department of Nephrology, University Hospital of Lund, Sweden.

Using a technique to acutely seal off various parts of the peritoneal membrane surface, with or without evisceration, we investigated the role of diaphragmatic, visceral, and parietal peritoneal lymphatic pathways in the drainage of 125I-labeled albumin (RISA) from the peritoneal cavity to the plasma during acute peritoneal dialysis in artificially ventilated rats. The total RISA clearance out of the peritoneal cavity (Cl) as well as the portion of this Cl reaching the plasma per unit time (Cl--> P) were assessed. Under non-steady-state conditions, the Cl was fivefold higher than the Cl--> P. Evisceration caused a 25-30% reduction in both Cl--> P and Cl. Sealing of the diaphragm, however, reduced the Cl--> P by 55% without affecting the Cl. A further reduction in the Cl--> P was obtained by combining sealing of the diaphragm with evisceration, which again markedly reduced the Cl. However, the greatest reduction in the Cl was obtained when the peritoneal surfaces of the anterior abdominal wall were sealed off in eviscerated rats. The discrepancy between the Cl and the Cl--> P can be explained by the local entrance of fluid and macromolecules into periabdominal tissues, where fluid is rapidly absorbed through the capillary walls via the Starling forces, while macromolecules are accumulating due to their very slow uptake by tissue lymphatics under non-steady-state conditions. Of the portion of the total Cl that rapidly entered the plasma, conceivably by lymphatic absorption, 55% could be ascribed to diaphragmatic lymphatics 30% to visceral lymphatics, and only some 10-15% to parietal lymphatics.

This article has been cited by other articles:

				S. J. Lai-Fook, P. K. Houtz, and P. D. Jones Transdiaphragmatic transport of tracer albumin from peritoneal to pleural liquid measured in rats J Appl Physiol, December 1, 2005; 99(6): 2212 - 2221. [Abstract] [Full Text] [PDF]

				W. T. Phillips, L. A. Medina, R. Klipper, and B. Goins A Novel Approach for the Increased Delivery of Pharmaceutical Agents to Peritoneum and Associated Lymph Nodes J. Pharmacol. Exp. Ther., October 1, 2002; 303(1): 11 - 16. [Abstract] [Full Text] [PDF]

				E. R. Zakaria, J. Lofthouse, and M. F. Flessner Effect of intraperitoneal pressures on tissue water of the abdominal muscle Am J Physiol Renal Physiol, June 1, 2000; 278(6): F875 - F885. [Abstract] [Full Text] [PDF]

				O. Carlsson and B. Rippe Peritoneal lymphatic absorption and solute exchange during zymosan-induced peritonitis in the rat Am J Physiol Heart Circ Physiol, September 1, 1999; 277(3): H1107 - H1112. [Abstract] [Full Text] [PDF]

				E. R. Zakaria, J. Lofthouse, and M. F. Flessner In vivo effects of hydrostatic pressure on interstitium of abdominal wall muscle Am J Physiol Heart Circ Physiol, February 1, 1999; 276(2): H517 - H529. [Abstract] [Full Text] [PDF]

				E. R. Zakaria, J. Lofthouse, and M. F. Flessner In vivo hydraulic conductivity of muscle: effects of hydrostatic pressure Am J Physiol Heart Circ Physiol, December 1, 1997; 273(6): H2774 - H2782. [Abstract] [Full Text] [PDF]