| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Heart and Circulatory Physiology, Vol 273, Issue 3 1415-H1426, Copyright © 1997 by American Physiological Society
ARTICLES |
E. S. Silverman, L. M. Khachigian, V. Lindner, A. J. Williams and T. Collins
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
Platelet-derived growth factor (PDGF) A-chain is expressed by vascular smooth muscle cells (SMC) in a variety of pathological settings. Phorbol 12-myristate 13-acetate (PMA) increases A-chain transcription and was used as a model agonist. Transient transfection analysis identified a region in the promoter that is required for inducibility, located between base pairs -71 and -55 from the transcription start site. This region contains overlapping recognition elements for members of the Sp and Egr families. Egr-1 transcript and protein increased after PMA treatment, whereas Sp1 and Sp3 levels remain unchanged. Egr-1 expression and PDGF A-chain promoter activity also increased in cells exposed to PDGF or mechanical injury. In vitro binding assays demonstrated that Egr-1, Sp1, and Sp3 can bind to this promoter region and that increasing Egr-1 can displace both Sp1 and Sp3. In an in vivo model of arterial injury, Egr-1 expression was induced concurrently with the expression of PDGF-A in SMC. Displacement of Sp1 and Sp3 by Egr-1 is correlated with inducible PDGF A-chain expression in the vessel wall.
This article has been cited by other articles:
|
|
 |
|
  J. Malakooti, R. Sandoval, V. C. Memark, P. K. Dudeja, and K. Ramaswamy Zinc finger transcription factor Egr-1 is involved in stimulation of NHE2 gene expression by phorbol 12-myristate 13-acetate Am J Physiol Gastrointest Liver Physiol, October 1, 2005; 289(4): G653 - G663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hjoberg, L. Le, A. Imrich, V. Subramaniam, S. I. Mathew, J. Vallone, K. J. Haley, F. H. Y. Green, S. A. Shore, and E. S. Silverman Induction of early growth-response factor 1 by platelet-derived growth factor in human airway smooth muscle Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L817 - L825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Naraba, C. Yokoyama, N. Tago, M. Murakami, I. Kudo, M. Fueki, S. Oh-ishi, and T. Tanabe Transcriptional Regulation of the Membrane-associated Prostaglandin E2 Synthase Gene. ESSENTIAL ROLE OF THE TRANSCRIPTION FACTOR Egr-1 J. Biol. Chem., August 2, 2002; 277(32): 28601 - 28608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Storm van's Gravesande, M. D. Layne, Q. Ye, L. Le, R. M. Baron, M. A. Perrella, L. Santambrogio, E. S. Silverman, and R. J. Riese IFN Regulatory Factor-1 Regulates IFN-{gamma}-Dependent Cathepsin S Expression J. Immunol., May 1, 2002; 168(9): 4488 - 4494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Morimoto, N. Kume, S. Miyamoto, Y. Ueno, H. Kataoka, M. Minami, K. Hayashida, N. Hashimoto, and T. Kita Lysophosphatidylcholine Induces Early Growth Response Factor-1 Expression and Activates the Core Promoter of PDGF-A Chain in Vascular Endothelial Cells Arterioscler. Thromb. Vasc. Biol., May 1, 2001; 21(5): 771 - 776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhang, S. D. Yan, A. Zhu, Y. S. Zou, M. Williams, G. C. Godman, B. M. Thomashow, M. E. Ginsburg, D. M. Stern, and S.-F. Yan Expression of Egr-1 in Late Stage Emphysema Am. J. Pathol., October 1, 2000; 157(4): 1311 - 1320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-F. Yan, J. Lu, L. Xu, Y. S. Zou, J. Tongers, W. Kisiel, N. Mackman, D. J. Pinsky, and D. M. Stern Pulmonary expression of early growth response-1: biphasic time course and effect of oxygen concentration J Appl Physiol, June 1, 2000; 88(6): 2303 - 2309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Vidal, J. Aragones, A. Alfranca, and M. O. de Landazuri Up-regulation of vascular endothelial growth factor receptor Flt-1 after endothelial denudation: role of transcription factor Egr-1 Blood, June 1, 2000; 95(11): 3387 - 3395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Saadane, L. Alpert, and L. E. Chalifour Altered molecular response to adrenoreceptor-induced cardiac hypertrophy in Egr-1-deficient mice Am J Physiol Heart Circ Physiol, March 1, 2000; 278(3): H796 - H805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. SILVERMAN and J. M. DRAZEN Genetic Variations in the 5-Lipoxygenase Core Promoter . Description and Functional Implications Am. J. Respir. Crit. Care Med., February 1, 2000; 161(2): S77 - 80. [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Heldin and B. Westermark Mechanism of Action and In Vivo Role of Platelet-Derived Growth Factor Physiol Rev, October 1, 1999; 79(4): 1283 - 1316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Miano and B. C. Berk NAB2: A Transcriptional Brake for Activated Gene Expression in the Vessel Wall? Am. J. Pathol., October 1, 1999; 155(4): 1009 - 1012. [Full Text] [PDF]
|
|
|
|
|
|
E. S. Silverman, L. M. Khachigian, F. S. Santiago, A. J. Williams, V. Lindner, and T. Collins Vascular Smooth Muscle Cells Express the Transcriptional Corepressor NAB2 in Response to Injury Am. J. Pathol., October 1, 1999; 155(4): 1311 - 1317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. O'Donovan and J. M. Baraban Major Egr3 Isoforms Are Generated via Alternate Translation Start Sites and Differ in Their Abilities To Activate Transcription Mol. Cell. Biol., July 1, 1999; 19(7): 4711 - 4718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Silverman and T. Collins Pathways of Egr-1-Mediated Gene Transcription in Vascular Biology Am. J. Pathol., March 1, 1999; 154(3): 665 - 670. [Full Text] [PDF]
|
|
|
|
|
|
B. K. Dieckgraefe and D. M. Weems Epithelial injury induces Egr-1 and Fos expression by a pathway involving protein kinase C and ERK Am J Physiol Gastrointest Liver Physiol, February 1, 1999; 276(2): G322 - G330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Du, C. Fu, K. C. Kent, H. Bush Jr., A. H. Schulick, K. Kreiger, T. Collins, and T. A. McCaffrey Elevated Egr-1 in Human Atherosclerotic Cells Transcriptionally Represses the Transforming Growth Factor-beta Type II Receptor J. Biol. Chem., December 8, 2000; 275(50): 39039 - 39047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Connor, I. Irrcher, and D. A. Hood Contractile Activity-induced Transcriptional Activation of Cytochrome c Involves Sp1 and Is Proportional to Mitochondrial ATP Synthesis in C2C12 Muscle Cells J. Biol. Chem., May 4, 2001; 276(19): 15898 - 15904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Liu, M. S. Prenger, D. D. Norton, L. Mei, J. W. Kusiak, and G. Bai Nerve Growth Factor Uses Ras/ERK and Phosphatidylinositol 3-Kinase Cascades to Up-regulate the N-Methyl-D-aspartate Receptor 1 Promoter J. Biol. Chem., November 21, 2001; 276(48): 45372 - 45379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. P. Quinn, M. Schlueter, S. J. Soifer, and J. A. Gutierrez Mechanotransduction in the Lung: Cyclic mechanical stretch induces VEGF and FGF-2 expression in pulmonary vascular smooth muscle cells Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L897 - L903. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
