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Am J Physiol Heart Circ Physiol 273: H2217-H2223, 1997;
0363-6135/97 $5.00
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Vol. 273, Issue 5, H2217-H2223, November 1997

Flow- and agonist-mediated nitric oxide- and prostaglandin-dependent dilation in spinal arteries

Yasuaki Yashiro and Toshio Ohhashi

The 1st Department of Physiology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390, Japan

Isolated rabbit spinal resistance-sized arteries (~100 µm in diameter and ~3 mm long) were cannulated at both ends with glass micropipettes and perfused at constant pressure (60 mmHg). An increase of flow rate corresponding to a change of pressure gradient (Delta P) ranging from 0 to 20 mmHg produced a flow-dependent vasodilation. Treatment with 50 µM aspirin or 10 µM indomethacin produced a significant reduction of the flow-dependent vasodilation only at Delta P of 5 mmHg. In contrast, treatment with Nomega -nitro-L-arginine methyl ester (L-NAME, 30 µM) produced no significant change. In the presence of 10 µM indomethacin, however, 30 µM L-NAME caused a marked decrease in the arterial diameter at Delta P of 5 mmHg, which was completely reversed with additional administration of 1 mM L-arginine. Acetylcholine (ACh) produced a dose-dependent increase in the arterial diameter. The ACh-induced vasodilation was significantly reduced by 10 µM indomethacin or 50 µM aspirin and partially suppressed by 30 µM L-NAME. Pretreatment with both indomethacin and L-NAME completely reduced the ACh-induced vasodilation. In the presence of 10 µM indomethacin, additional treatment with 1 mM L-arginine significantly reversed the L-NAME-induced inhibition of the ACh-mediated vasodilation. Endothelial removal with Triton X-100 significantly reduced the ACh-induced vasodilation. Isocarbacyclin (a stable prostaglandin I2 analogue), prostaglandin E2, and arachidonic acid caused a dose-dependent dilation in the small arteries. These findings suggest that prostanoids play a major role in the flow- or ACh-induced vasodilation in the rabbit spinal resistance-sized small arteries.

spine; resistance-sized small artery; shear stress; acetylcholine; endothelium-derived relaxing factors


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