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Am J Physiol Heart Circ Physiol 273: H2867-H2875, 1997;
0363-6135/97 $5.00
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Vol. 273, Issue 6, H2867-H2875, December 1997

Effect of a 14-day hindlimb suspension on cytosolic Ca2+ concentration in rat portal vein myocytes

Jean-Luc Morel, François-Xavier Boittin, Guillaume Halet, Serge Arnaudeau, Chantal Mironneau, and Jean Mironneau

Laboratoire de Physiologie Cellulaire et Pharmacologie Moléculaire, Centre National de la Recherche Scientifique ESA 5017, Université de Bordeaux II, 33076 Bordeaux, France

Effects of a 14-day hindlimb suspension were examined on increases in cytosolic Ca2+ concentration ([Ca2+]i) evoked by vasoactive compounds and on Ca2+ channels in rat portal vein myocytes. The maximal increases in [Ca2+]i elicited by caffeine, norepinephrine, and angiotensin II were reduced by 30-50% in suspended rats, and complete recovery was obtained 4 days after suspension removal. In contrast, voltage-gated Ca2+ channels were unaffected by hindlimb suspension. Using both confocal microscopy and the patch-clamp technique, we measured local increases in [Ca2+]i which corresponded to activation of a small number of ryanodine-sensitive Ca2+-release channels (Ca2+ sparks) and D-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]-gated Ca2+ channels. After hindlimb suspension, these local Ca2+ events, as well as the Ca2+ sensitivity of ryanodine-sensitive Ca2+ release channels, remained unchanged. In contrast, the propagated Ca2+ responses (Ca2+ waves) were significantly reduced in parallel with a noticeable inhibition of [3H]ryanodine binding to vascular membranes. Taken together, these results suggest that inhibition of the vasoconstrictor-induced increases in [Ca2+]i during long-term suspension may be related to a reduction of the number of functional ryanodine-sensitive and Ins(1,4,5)P3-gated channels in the sarcoplasmic reticulum of rat portal vein myocytes.

rat vascular smooth muscle; confocal microscopy; calcium release channel; D-myo-inositol 1,4,5-trisphosphate-gated channel; ryanodine


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