| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-subunit regulation in aortic smooth muscle cells
1 Department of Pharmacology
and Experimental Therapeutics,
This study was designed to test the role of
Na+ and
Ca2+ entry in the stretch-induced
Na+-K+-ATPase
1- and
2-isoform upregulation observed
in our previous studies. We measured intracellular
Na+ in cyclically stretched rat
aortic smooth muscle cells, with or without gadolinium treatment, for
various durations and performed Western blotting to analyze the effects
of stretch and the calcium channel blocker isradipine on the expression
of -isoforms. Intracellular Na+
was elevated significantly after 1- and 2-h stretch, but returned to
baseline after 1-, 2-, and 4-day stretch. This increase in intracellular Na+ was blocked by
gadolinium. Both 1- and
2-isoforms were upregulated after either 2 or 4 days of cyclical stretch. Isradipine had no apparent effect on stretch-induced upregulation on either -isoform, thus suggesting that Ca2+ entry
through L-type channels is not involved in the stretch-induced upregulation. We therefore conclude that a transient intracellular Na+ elevation during stretch may
serve as a signal to mediate the 1- and
2-isoform upregulation.
vascular smooth muscle cells; sodium-potassium-adenosinetriphosphatase -isoforms; isradipine; intracellular sodium; mechanical strain
This article has been cited by other articles:
|
|
 |
|
  E. Songu-Mize, N. Sevieux, X. Liu, and M. Jacobs Effect of short-term cyclic stretch on sodium pump activity in aortic smooth muscle cells Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H2072 - H2078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Lavelin, N. Meiri, O. Genina, R. Alexiev, and M. Pines Na+-K+-ATPase gene expression in the avian eggshell gland: distinct regulation in different cell types Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2001; 281(4): R1169 - R1176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sevieux, J. Alam, and E. Songu-Mize Effect of cyclic stretch on {alpha}-subunit mRNA expression of Na+-K+-ATPase in aortic smooth muscle cells Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1555 - C1560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aydemir-Koksoy and J. C. Allen Regulation of Na+ pump expression by vascular smooth muscle cells Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1869 - H1874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Iwasaki, S. Eguchi, H. Ueno, F. Marumo, and Y. Hirata Mechanical stretch stimulates growth of vascular smooth muscle cells via epidermal growth factor receptor Am J Physiol Heart Circ Physiol, February 1, 2000; 278(2): H521 - H529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R. Standley, T. J. Obards, and C. L. Martina Cyclic stretch regulates autocrine IGF-I in vascular smooth muscle cells: implications in vascular hyperplasia Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E697 - E705. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
