| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Cox Laboratory for Biomedical
Engineering,
This study demonstrated that exposure of cultured human aortic smooth muscle cells (SMC) to fluid flow resulted in nitric oxide (NO) production, monitored by nitrite and guanosine 3',5'-cyclic monophosphate production. A rapid burst in nitrite production rate was followed by a more gradual increase throughout the period of flow exposure. Neither the initial burst nor the prolonged nitrite production was dependent on the level of shear stress in the range of 1.1-25 dyn/cm2. Repeated exposure to shear stress after a 30-min static period restimulated nitrite production similar to the initial burst. Ca2+-calmodulin antagonists blocked the initial burst in nitrite release. An inhibitor of nitric oxide synthase (NOS) blocked nitrite production, indicating that changes in nitrite reflect NO production. Treatment with dexamethasone or cycloheximide had no effect on nitrite production. Monoclonal antibodies directed against the inducible and endothelial NOS isoforms showed no immunoreactivity on Western blots, whereas monoclonal antibodies directed against the neuronal NOS gave specific products. These findings suggest that human aortic SMC express a constitutive neuronal NOS isoform, the enzymatic activity of which is modulated by flow.
neuronal nitric oxide synthase; shear stress
This article has been cited by other articles:
|
|
 |
|
  F. S. Michel, R. Y. K. Man, and P. M. Vanhoutte Increased spontaneous tone in renal arteries of spontaneously hypertensive rats Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1673 - H1681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Han, H. Ma, R. Chintala, K. Miyake, D. J. R. Fulton, S. A. Barman, and R. E. White Nongenomic, endothelium-independent effects of estrogen on human coronary smooth muscle are mediated by type I (neuronal) NOS and PI3-kinase-Akt signaling Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H314 - H321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Garanich, M. Pahakis, and J. M. Tarbell Shear stress inhibits smooth muscle cell migration via nitric oxide-mediated downregulation of matrix metalloproteinase-2 activity Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2244 - H2252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ganguli, L. Persson, I. R. Palmer, I. Evans, L. Yang, R. Smallwood, R. Black, and E. E. Qwarnstrom Distinct NF-{kappa}B Regulation by Shear Stress Through Ras-Dependent I{kappa}B{alpha} Oscillations: Real-Time Analysis of Flow-Mediated Activation in Live Cells Circ. Res., April 1, 2005; 96(6): 626 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Ainslie, J. S. Garanich, R. O. Dull, and J. M. Tarbell Vascular smooth muscle cell glycocalyx influences shear stress-mediated contractile response J Appl Physiol, January 1, 2005; 98(1): 242 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Osanai, N. Akutsu, N. Fujita, T. Nakano, K. Takahashi, W. Guan, and K. Okumura Cross talk between prostacyclin and nitric oxide under shear in smooth muscle cell: role in monocyte adhesion Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H177 - H182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Gosgnach, D. Messika-Zeitoun, W. Gonzalez, M. Philipe, and J.-B. Michel Shear stress induces iNOS expression in cultured smooth muscle cells: role of oxidative stress Am J Physiol Cell Physiol, December 1, 2000; 279(6): C1880 - C1888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wang and J. M. Tarbell Effect of Fluid Flow on Smooth Muscle Cells in a 3-Dimensional Collagen Gel Model Arterioscler Thromb Vasc Biol, October 1, 2000; 20(10): 2220 - 2225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Cai, J. Xin, D. M. Pollock, and J. S. Pollock Shear stress-mediated NO production in inner medullary collecting duct cells Am J Physiol Renal Physiol, August 1, 2000; 279(2): F270 - F274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tada and J. M. Tarbell Interstitial flow through the internal elastic lamina affects shear stress on arterial smooth muscle cells Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1589 - H1597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Brophy, L. Knoepp, J. Xin, and J. S. Pollock Functional expression of NOS 1 in vascular smooth muscle Am J Physiol Heart Circ Physiol, March 1, 2000; 278(3): H991 - H997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Rhoads, S. G. Eskin, and L. V. McIntire Fluid Flow Releases Fibroblast Growth Factor-2 From Human Aortic Smooth Muscle Cells Arterioscler Thromb Vasc Biol, February 1, 2000; 20(2): 416 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Schwarz, H. Kleinert, and U. Forstermann Potential Functional Significance of Brain-Type and Muscle-Type Nitric Oxide Synthase I Expressed in Adventitia and Media of Rat Aorta Arterioscler Thromb Vasc Biol, November 1, 1999; 19(11): 2584 - 2590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Marano, S. Palazzesi, A. Vergari, and A. U. Ferrari Protection by Shear Stress From Collar-Induced Intimal Thickening : Role of Nitric Oxide Arterioscler Thromb Vasc Biol, November 1, 1999; 19(11): 2609 - 2614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. L. Day, L. A. Rafty, C. N. Chesterman, and L. M. Khachigian Angiotensin II (ATII)-inducible Platelet-derived Growth Factor A-chain Gene Expression Is p42/44 Extracellular Signal-regulated Kinase-1/2 and Egr-1-dependent and Mediated via the ATII Type 1 but Not Type 2 Receptor. INDUCTION BY ATII ANTAGONIZED BY NITRIC OXIDE J. Biol. Chem., August 20, 1999; 274(34): 23726 - 23733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tada and J. M. Tarbell Flow through internal elastic lamina affects shear stress on smooth muscle cells (3D simulations) Am J Physiol Heart Circ Physiol, February 1, 2002; 282(2): H576 - H584. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
