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Department of Bioengineering, University of California-San Diego, La Jolla, California 92093
The events
responsible for cell injury after a tissue stimulation are only
incompletely understood. The purpose of this study was to examine
mechanisms of cell injury in two tissues, rat mesentery and cremaster
muscle, after tissue stimulation with
N-formylmethionyl-leucyl-phenylalanine (FMLP) and
platelet-activating factor (PAF). The response was studied in the same
animal in random order using normal and leukopenic rats. The tissues
were exteriorized after pentobarbital anesthesia. Five to six
vascularized areas were chosen in each tissue, and cell injury and
hydroperoxide production were assessed visually by continuous
superfusion with 1 µM propidium iodide and 5 µM dichlorofluorescin
diacetate (DCFH), respectively. FMLP (1 × 10
8 M) and then PAF (1 × 108 M) were added
to the superfusate, and measurements were made at several time points.
The second tissue was then examined using the same protocol. In the
cremaster, there was little hydroperoxide production, and the tissue
injury was eliminated after leukopenia. Leukopenia had no effect on
tissue injury in the mesentery. Although hydroperoxide production was
observed, there was no correlation between it and the tissue injury.
The level of preactivation showed no correlation with either tissue
injury or hydroperoxide production. In light of these results, mast
cell degranulation may be an important mechanism of tissue injury in
the mesentery.
microcirculation; N-formylmethionyl-leucyl-phenylalanine; platelet-activating factor; propidium iodide; dichlorofluorescin diacetate
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