| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Medicine and Division of Physiology, Schools of Medicine and Public Health, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287
The mouse is the species of choice for
creating genetically engineered models of human disease. To study
detailed systolic and diastolic left ventricular (LV) chamber mechanics
in mice in vivo, we developed a miniaturized conductance-manometer
system. 
-Chloralose-urethan-anesthetized animals were instrumented
with a two-electrode pressure-volume catheter advanced via the LV apex to the aortic root. Custom electronics provided time-varying
conductances related to cavity volume. Baseline hemodynamics were
similar to values in conscious animals: 634 ± 14 beats/min, 112 ± 4 mmHg, 5.3 ± 0.8 mmHg, and 11,777 ± 732 mmHg/s for heart
rate, end-systolic and end-diastolic pressures, and
maximum first derivative of ventricular pressure with respect to time
(dP/dtmax),
respectively. Catheter stroke volume during preload reduction by
inferior vena caval occlusion correlated with that by ultrasound aortic
flow probe (r2 = 0.98). This maneuver yielded end-systolic elastances of 79 ± 21 mmHg/µl, preload-recruitable stroke work of 82 ± 5.6 mmHg, and
slope of
dP/dtmax-end-diastolic
volume relation of 699 ± 100 mmHg · s
1 · µl1,
and these relations varied predictably with acute inotropic interventions. The control normalized time-varying elastance curve was
similar to human data, further supporting comparable chamber mechanics
between species. This novel approach should greatly help assess
cardiovascular function in the blood-perfused murine heart.
mouse; ventricular function; hemodynamics; conductance volumetry
This article has been cited by other articles:
|
|
 |
|
  B. Yoo, A. Lemaire, S. Mangmool, M. J. Wolf, A. Curcio, L. Mao, and H. A. Rockman {beta}1-Adrenergic receptors stimulate cardiac contractility and CaMKII activation in vivo and enhance cardiac dysfunction following myocardial infarction Am J Physiol Heart Circ Physiol, October 1, 2009; 297(4): H1377 - H1386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Naghshin, K. R. McGaffin, W. G. Witham, M. A. Mathier, L. C. Romano, S. H. Smith, A. M. Janczewski, J. A. Kirk, S. G. Shroff, and C. P. O'Donnell Chronic intermittent hypoxia increases left ventricular contractility in C57BL/6J mice J Appl Physiol, September 1, 2009; 107(3): 787 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Radovits, S. Korkmaz, S. Loganathan, E. Barnucz, T. Bomicke, R. Arif, M. Karck, and G. Szabo Comparative investigation of the left ventricular pressure-volume relationship in rat models of type 1 and type 2 diabetes mellitus Am J Physiol Heart Circ Physiol, July 1, 2009; 297(1): H125 - H133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Patten and M. R. Hall-Porter Small Animal Models of Heart Failure: Development of Novel Therapies, Past and Present Circ Heart Fail, March 1, 2009; 2(2): 138 - 144. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Donaldson, S. Eder, C. Baker, M. J. Aronovitz, A. D. Weiss, M. Hall-Porter, F. Wang, A. Ackerman, R. H. Karas, J. D. Molkentin, et al. Estrogen Attenuates Left Ventricular and Cardiomyocyte Hypertrophy by an Estrogen Receptor-Dependent Pathway That Increases Calcineurin Degradation Circ. Res., January 30, 2009; 104(2): 265 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Chantler, E. G. Lakatta, and S. S. Najjar Arterial-ventricular coupling: mechanistic insights into cardiovascular performance at rest and during exercise J Appl Physiol, October 1, 2008; 105(4): 1342 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Shioura, D. L. Geenen, and P. H. Goldspink Sex-related changes in cardiac function following myocardial infarction in mice Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R528 - R534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Tankersley, H. C. Champion, E. Takimoto, K. Gabrielson, D. Bedja, V. Misra, H. El-Haddad, R. Rabold, and W. Mitzner Exposure to inhaled particulate matter impairs cardiac function in senescent mice Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2008; 295(1): R252 - R263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Bal, W. B. de Vries, M. F. M. van Oosterhout, J. Baan, E. E. van der Wall, F. van Bel, and P. Steendijk Long-term cardiovascular effects of neonatal dexamethasone treatment: hemodynamic follow-up by left ventricular pressure-volume loops in rats J Appl Physiol, February 1, 2008; 104(2): 446 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Shioura, D. L. Geenen, and P. H. Goldspink Assessment of cardiac function with the pressure-volume conductance system following myocardial infarction in mice Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2870 - H2877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liu, J. A. Simpson, K. R. Brunt, C. A. Ward, S. R. R. Hall, R. T. Kinobe, V. Barrette, M. Y. Tse, S. C. Pang, A. S. Pachori, et al. Preemptive heme oxygenase-1 gene delivery reveals reduced mortality and preservation of left ventricular function 1 yr after acute myocardial infarction Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H48 - H59. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Nielsen, S. B. Kristiansen, S. Ringgaard, T. T. Nielsen, A. Flyvbjerg, A. N. Redington, and H. E. Botker Left ventricular volume measurement in mice by conductance catheter: evaluation and optimization of calibration Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H534 - H540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Jegger, A. S. Mallik, M. Nasratullah, X. Jeanrenaud, R. d. Silva, H. Tevaearai, L. K. von Segesser, and N. Stergiopulos The effect of a myocardial infarction on the normalized time-varying elastance curve J Appl Physiol, March 1, 2007; 102(3): 1123 - 1129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Joho, S. Ishizaka, R. Sievers, E. Foster, P. C. Simpson, and W. Grossman Left ventricular pressure-volume relationship in conscious mice Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H369 - H377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Bilchick, J. G. Duncan, R. Ravi, E. Takimoto, H. C. Champion, W. D. Gao, L. B. Stull, D. A. Kass, and A. M. Murphy Heart failure-associated alterations in troponin I phosphorylation impair ventricular relaxation-afterload and force-frequency responses and systolic function Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H318 - H325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Reyes, M. E. Steinhelper, J. A. Alvarez, D. Escobedo, J. Pearce, J. W. Valvano, B. H. Pollock, C.-L. Wei, A. Kottam, D. Altman, et al. Impact of physiological variables and genetic background on myocardial frequency-resistivity relations in the intact beating murine heart Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1659 - H1669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Connelly, D. L. Prior, D. J. Kelly, M. P. Feneley, H. Krum, and R. E. Gilbert Load-sensitive measures may overestimate global systolic function in the presence of left ventricular hypertrophy: a comparison with load-insensitive measures Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1699 - H1705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. B Popovic, J. P. Sun, H. Yamada, J. Drinko, K. Mauer, N. L Greenberg, Y. Cheng, C. S Moravec, M. S Penn, T. N Mazgalev, et al. Differences in left ventricular long-axis function from mice to humans follow allometric scaling to ventricular size J. Physiol., October 1, 2005; 568(1): 255 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Burkhoff, I. Mirsky, and H. Suga Assessment of systolic and diastolic ventricular properties via pressure-volume analysis: a guide for clinical, translational, and basic researchers Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H501 - H512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O.-J. How, E. Aasum, S. Kunnathu, D. L. Severson, E. S. P. Myhre, and T. S. Larsen Influence of substrate supply on cardiac efficiency, as measured by pressure-volume analysis in ex vivo mouse hearts Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2979 - H2985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Kassab, E. R. Lontis, A. Horlyck, and H. Gregersen Novel method for measurement of medium size arterial lumen area with an impedance catheter: in vivo validation Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H2014 - H2020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Segers, D. Georgakopoulos, M. Afanasyeva, H. C. Champion, D. P. Judge, H. D. Millar, P. Verdonck, D. A. Kass, N. Stergiopulos, and N. Westerhof Conductance catheter-based assessment of arterial input impedance, arterial function, and ventricular-vascular interaction in mice Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1157 - H1164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Ikonomidis, J. W. Hendrick, A. M. Parkhurst, A. R. Herron, P. G. Escobar, K. B. Dowdy, R. E. Stroud, E. Hapke, M. R. Zile, and F. G. Spinale Accelerated LV remodeling after myocardial infarction in TIMP-1-deficient mice: effects of exogenous MMP inhibition Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H149 - H158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pacher, J. G. Mabley, L. Liaudet, O. V. Evgenov, A. Marton, G. Hasko, M. Kollai, and C. Szabo Left ventricular pressure-volume relationship in a rat model of advanced aging-associated heart failure Am J Physiol Heart Circ Physiol, November 1, 2004; 287(5): H2132 - H2137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A Alvarez, M. Reyes, D. Escobedo, G. L Freeman, M. E Steinhelper, and M. D Feldman Enhanced left ventricular systolic function early in type 2 diabetic mice: clinical implications Diabetes and Vascular Disease Research, October 1, 2004; 1(2): 89 - 94. [Abstract] [PDF]
|
|
|
|
|
|
B. M. Palmer, D. Georgakopoulos, P. M. Janssen, Y. Wang, N. R. Alpert, D. F. Belardi, S. P. Harris, R. L. Moss, P. G. Burgon, C. E. Seidman, et al. Role of Cardiac Myosin Binding Protein C in Sustaining Left Ventricular Systolic Stiffening Circ. Res., May 14, 2004; 94(9): 1249 - 1255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Soergel, D. Georgakopoulos, L. B. Stull, D. A. Kass, and A. M. Murphy Augmented systolic response to the calcium sensitizer EMD-57033 in a transgenic model with troponin I truncation Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1785 - H1792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Khairallah, F. Labarthe, B. Bouchard, G. Danialou, B. J. Petrof, and C. Des Rosiers Profiling substrate fluxes in the isolated working mouse heart using 13C-labeled substrates: focusing on the origin and fate of pyruvate and citrate carbons Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1461 - H1470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ishizaka, R. E. Sievers, B.-Q. Zhu, M. C. Rodrigo, S. Joho, E. Foster, P. C. Simpson, and W. Grossman New technique for measurement of left ventricular pressure in conscious mice Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H1208 - H1215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang, H. R. Brunner, and M. Burnier Determination of cardiac contractility in awake unsedated mice with a fluid-filled catheter Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H806 - H814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Collins, C. E. Korcarz, and R. M. Lang Use of echocardiography for the phenotypic assessment of genetically altered mice Physiol Genomics, May 13, 2003; 13(3): 227 - 239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. J. M. Creemers, J. N. Davis, A. M. Parkhurst, P. Leenders, K. B. Dowdy, E. Hapke, A. M. Hauet, P. G. Escobar, J. P. M. Cleutjens, J. F. M. Smits, et al. Deficiency of TIMP-1 exacerbates LV remodeling after myocardial infarction in mice Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H364 - H371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nemoto, G. DeFreitas, D. L. Mann, and B. A. Carabello Effects of changes in left ventricular contractility on indexes of contractility in mice Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2504 - H2510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Semeniuk, A. J. Kryski, and D. L. Severson Echocardiographic assessment of cardiac function in diabetic db/db and transgenic db/db-hGLUT4 mice Am J Physiol Heart Circ Physiol, September 1, 2002; 283(3): H976 - H982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. A. Janssen and J. F. M. Smits Autonomic control of blood pressure in mice: basic physiology and effects of genetic modification Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1545 - R1564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Lorenz A practical guide to evaluating cardiovascular, renal, and pulmonary function in mice Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1565 - R1582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Liang, E. C. Carlson, R. V. Donthi, P. M. Kralik, X. Shen, and P. N. Epstein Overexpression of Metallothionein Reduces Diabetic Cardiomyopathy Diabetes, January 1, 2002; 51(1): 174 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. T. Hart, J. C. Burnett Jr., and M. M. Redfield Effects of avertin versus xylazine-ketamine anesthesia on cardiac function in normal mice Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H1938 - H1945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Belke, T. S. Larsen, E. M. Gibbs, and D. L. Severson Altered metabolism causes cardiac dysfunction in perfused hearts from diabetic (db/db) mice Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1104 - E1113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Feldman, J. M. Erikson, Y. Mao, C. E. Korcarz, R. M. Lang, and G. L. Freeman Validation of a mouse conductance system to determine LV volume: comparison to echocardiography and crystals Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1698 - H1707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Paolocci, U. E. G. Ekelund, T. Isoda, M. Ozaki, K. Vandegaer, D. Georgakopoulos, R. W. Harrison, D. A. Kass, and J. M. Hare cGMP-independent inotropic effects of nitric oxide and peroxynitrite donors: potential role for nitrosylation Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1982 - H1988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Feldman, Y. Mao, J. W. Valvano, J. A. Pearce, and G. L. Freeman Development of a multifrequency conductance catheter-based system to determine LV function in mice Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H1411 - H1420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Georgakopoulos and D. A. Kass Estimation of parallel conductance by dual-frequency conductance catheter in mice Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H443 - H450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Yang, D. F. Larson, and R. Watson Age-related left ventricular function in the mouse: analysis based on in vivo pressure-volume relationships Am J Physiol Heart Circ Physiol, November 1, 1999; 277(5): H1906 - H1913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Belke, T. S. Larsen, G. D. Lopaschuk, and D. L. Severson Glucose and fatty acid metabolism in the isolated working mouse heart Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1999; 277(4): R1210 - R1217. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
