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Am J Physiol Heart Circ Physiol 274: H1443-H1449, 1998;
0363-6135/98 $5.00
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Vol. 274, Issue 5, H1443-H1449, May 1998

Inhibition of myocardial glucose uptake by cGMP

Christophe Depre1,2, Vinciane Gaussin1, Sylvie Ponchaut1, Yvan Fischer3, Jean-Louis Vanoverschelde2, and Louis Hue1

1 Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, 2 Division of Cardiology, Louvain University Medical School, B-1200 Brussels, Belgium; and 3 Institute of Physiology, Medical Faculty, Rheinisch-Westfälische Technische Hochschule, D-52057 Aachen, Germany

Guanosine 3',5'-cyclic monophosphate (cGMP), a second messenger of nitric oxide (NO), regulates myocardial contractility. It is not known whether this effect is accompanied by a change in heart metabolism. We report here the effects of 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP), a cGMP analog, on regulatory steps of glucose metabolism in isolated working rat hearts perfused with glucose as the substrate. When glucose uptake was stimulated by increasing the workload, addition of the cGMP analog totally suppressed this stimulation and accelerated net glycogen breakdown. 8-BrcGMP did not affect pyruvate dehydrogenase activity but activated acetyl-CoA carboxylase, the enzyme that produces malonyl-CoA, an inhibitor of long-chain fatty acid oxidation. To test whether glucose metabolism could also be affected by altering the intracellular concentration of cGMP, we perfused hearts with NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of NO synthase, or with S-nitroso-N-acetylpenicillamine (SNAP), a NO donor. Perfusion with L-NAME decreased cGMP and increased glucose uptake by 30%, whereas perfusion with SNAP resulted in opposite effects. None of these conditions affected adenosine 3',5'-cyclic monophosphate concentration. Limitation of glucose uptake by SNAP or 8-BrcGMP decreased heart work, and this was reversed by adding alternative oxidizable substrates (pyruvate, beta -hydroxybutyrate) together with glucose. Therefore, increased NO production decreases myocardial glucose utilization and limits heart work. This effect is mediated by cGMP, which is thus endowed with both physiological and metabolic properties.

glycolysis; glucose uptake; nitric oxide; working heart


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