AJP - Heart Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 274: H1928-H1932, 1998;
0363-6135/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Inoue, N.
Right arrow Articles by Yokoyama, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Inoue, N.
Right arrow Articles by Yokoyama, M.
Vol. 274, Issue 6, H1928-H1932, June 1998

Stretch force on vascular smooth muscle cells enhances oxidation of LDL via superoxide production

Nobutaka Inoue, Seinosuke Kawashima, Ken-Ichi Hirata, Yoshiyuki Rikitake, Saori Takeshita, Wataru Yamochi, Hozuka Akita, and Mitsuhiro Yokoyama

First Department of Internal Medicine, Kobe University School of Medicine, Kobe 650, Japan

Hemodynamic forces on vasculature profoundly influence atherogenesis. We examined the effect of stretch force on the oxidation of low-density lipoprotein (LDL) by rat aortic smooth muscle cells (RASM) and superoxide production. Stretch force was imposed on RASM cultured on deformable dishes by stretching the dishes. Incubation of native LDL with static RASM for 24 h resulted in LDL oxidation as indicated by increases in thiobarbituric acid-reacting substances from 9.5 ± 2.3 to 24.5 ± 2.3 nmol malondialdehyde/mg. Stretch force on RASM augmented cell-mediated LDL oxidation to 149.3 ± 17.1% concomitantly with increase in superoxide production. LDL oxidation was inhibited by superoxide dismutase or depletion of the metal ion in the culture medium, indicating that it was a metal ion-dependent and superoxide-mediated process. The enhancement of LDL oxidation by stretch force was inhibited by diphenyliodonium, indicating the involvement of the NADH/NADPH oxidase system. Our findings suggest that the increased oxidant stress induced by stretch force is one of the potential mechanisms whereby hypertension facilitates atherosclerosis.

hemodynamic force; oxidant stress; reduced nicotinamide adenine dinucleotide/reduced nicotinamide adenine dinucleotide phosphate oxidase; atherosclerosis; hypertension


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. E. Chapman, S. E. Sinclair, D. Zhuang, A. Hassid, L. P. Desai, and C. M. Waters
Cyclic mechanical strain increases reactive oxygen species production in pulmonary epithelial cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L834 - L841.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Q. Liu, J. S. K. Sham, L. A. Shimoda, P. Kuppusamy, and J. T. Sylvester
Hypoxic constriction and reactive oxygen species in porcine distal pulmonary arteries
Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L322 - L333.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online