| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Center, and Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada R2H 2A6; and Second Department of Internal Medicine, Yamanashi Medical University, Yamanashi, Japan 409-3898
To examine the effects of ischemic preconditioning on ischemia-reperfusion-induced changes in the sarcoplasmic reticulum (SR) function, isolated rat hearts were either perfused with a control medium for 30 min or preconditioned with three episodes of 5-min ischemia and 5-min reperfusion before sustained ischemia for 30 min followed by reperfusion for 30 min was induced. Preconditioning itself depressed cardiac function (left ventricular developed pressure, peak rate of contraction, and peak rate of relaxation) and SR Ca2+-release and -uptake activities as well as protein content and Ca2+/calmodulin-dependent protein kinase (CaMK) phosphorylation of Ca2+-release channels by 25-60%. Global ischemia for 30 min produced marked depressions in SR Ca2+-release and -uptake activities as well as SR Ca2+-pump protein content in control hearts; these changes were significantly attenuated by preconditioning. Compared with the control preparations, preconditioning improved the recovery of cardiac function and SR Ca2+-release and -uptake activities as well as Ca2+-release channel and Ca2+-pump protein contents in the ischemic-reperfused hearts. Unlike the protein kinase A-mediated phosphorylation in SR membranes, the CaMK-mediated phosphorylations at Ca2+-release channels, Ca2+ pump, and phospholamban were depressed in the ischemic hearts; these changes were prevented by preconditioning. These results indicate that ischemic preconditioning may exert beneficial effects on ischemia-reperfusion-induced alterations in SR function by preventing changes in Ca2+-release channel and Ca2+-pump protein contents in the SR membrane.
calcium/calmodulin-dependent protein kinase phosphorylation; protein kinase A phosphorylation; sarcoplasmic reticulum calcium release; sarcoplasmic reticulum calcium uptake; sarcoplasmic reticulum phospholamban
This article has been cited by other articles:
|
|
 |
|
  Z. Yu, Z.-H. Wang, and H.-T. Yang Calcium/calmodulin-dependent protein kinase II mediates cardioprotection of intermittent hypoxia against ischemic-reperfusion-induced cardiac dysfunction Am J Physiol Heart Circ Physiol, August 1, 2009; 297(2): H735 - H742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wang, J.-F. Du, F. Wu, and H.-C. Wang Apelin decreases the SR Ca2+ content but enhances the amplitude of [Ca2+]i transient and contractions during twitches in isolated rat cardiac myocytes Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2540 - H2546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Rodrigo and N. J. Samani Ischemic preconditioning of the whole heart confers protection on subsequently isolated ventricular myocytes Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H524 - H531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Yeung, G. M. Kravtsov, K. M. Ng, T. M. Wong, and M. L. Fung Chronic intermittent hypoxia alters Ca2+ handling in rat cardiomyocytes by augmented Na+/Ca2+ exchange and ryanodine receptor activities in ischemia-reperfusion Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2046 - C2056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jiang, A. Xu, and N. Narayanan Thyroid hormone downregulates the expression and function of sarcoplasmic reticulum-associated CaM kinase II in the rabbit heart Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1384 - H1394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chen, X.-Y. Lu, J. Li, J.-D. Fu, Z.-N. Zhou, and H.-T. Yang Intermittent hypoxia protects cardiomyocytes against ischemia-reperfusion injury-induced alterations in Ca2+ homeostasis and contraction via the sarcoplasmic reticulum and Na+/Ca2+ exchange mechanisms Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1221 - C1229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mattiazzi, C. Mundina-Weilenmann, C. Guoxiang, L. Vittone, and E. Kranias Role of phospholamban phosphorylation on Thr17 in cardiac physiological and pathological conditions Cardiovasc Res, December 1, 2005; 68(3): 366 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamamura, C. Steenbergen, and E. Murphy Protein kinase C and preconditioning: role of the sarcoplasmic reticulum Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2484 - H2490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Takeda, S. Mochizuki, H. K. Saini, V. Elimban, and N. S. Dhalla Modification of alterations in cardiac function and sarcoplasmic reticulum by vanadate in ischemic-reperfused rat hearts J Appl Physiol, September 1, 2005; 99(3): 999 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xie, Y. Zhu, W.-Z. Zhu, L. Chen, Z.-N. Zhou, W.-J. Yuan, and H.-T. Yang Role of dual-site phospholamban phosphorylation in intermittent hypoxia-induced cardioprotection against ischemia-reperfusion injury Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2594 - H2602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Shao, B. Ren, H. K. Saini, T. Netticadan, N. Takeda, and N. S. Dhalla Sarcoplasmic reticulum Ca2+ transport and gene expression in congestive heart failure are modified by imidapril treatment Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1674 - H1682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jiang, A. Xu, D.L. Jones, and N. Narayanan Coordinate downregulation of CaM kinase II and phospholamban accompanies contractile phenotype transition in the hyperthyroid rabbit soleus Am J Physiol Cell Physiol, September 1, 2004; 287(3): C622 - C632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Pei, G. M. Kravtsov, S. Wu, R. Das, M. L. Fung, and T. M. Wong Calcium homeostasis in rat cardiomyocytes during chronic hypoxia: a time course study Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1420 - C1428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Temsah, T. Netticadan, K.-I. Kawabata, and N. S. Dhalla Lack of both oxygen and glucose contributes to I/R-induced changes in cardiac SR function Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1306 - C1312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. E. Louch, G. R. Ferrier, and S. E. Howlett Changes in excitation-contraction coupling in an isolated ventricular myocyte model of cardiac stunning Am J Physiol Heart Circ Physiol, August 1, 2002; 283(2): H800 - H810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Tupling and H. Green Silver ions induce Ca2+ release from the SR in vitro by acting on the Ca2+ release channel and the Ca2+ pump J Appl Physiol, April 1, 2002; 92(4): 1603 - 1610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Temsah, K. Kawabata, D. Chapman, and N. S. Dhalla Preconditioning prevents alterations in cardiac SR gene expression due to ischemia-reperfusion Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1461 - H1466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xu, M. V. Cohen, J. M. Downey, T. L. Vanden Hoek, and Z. Yao Attenuation of oxidant stress during reoxygenation by AMP 579 in cardiomyocytes Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2585 - H2589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Netticadan, R. M. Temsah, A. Kent, V. Elimban, and N. S. Dhalla Depressed Levels of Ca2+-Cycling Proteins May Underlie Sarcoplasmic Reticulum Dysfunction in the Diabetic Heart Diabetes, September 1, 2001; 50(9): 2133 - 2138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Tupling, H. Green, G. Senisterra, J. Lepock, and N. McKee Effects of ischemia on sarcoplasmic reticulum Ca2+ uptake and Ca2+ release in rat skeletal muscle Am J Physiol Endocrinol Metab, August 1, 2001; 281(2): E224 - E232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Rao, A. Xu, and N. Narayanan Glucocorticoid modulation of protein phosphorylation and sarcoplasmic reticulum function in rat myocardium Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H325 - H333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, G. Cherednichenko, L. Hernandez, J. Halow, S. A. Camacho, V. Figueredo, and S. Schaefer Preconditioning limits mitochondrial Ca2+ during ischemia in rat hearts: role of KATP channels Am J Physiol Heart Circ Physiol, May 1, 2001; 280(5): H2321 - H2328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Osada, T. Netticadan, K. Kawabata, K. Tamura, and N. S. Dhalla Ischemic preconditioning prevents I/R-induced alterations in SR calcium-calmodulin protein kinase II Am J Physiol Heart Circ Physiol, June 1, 2000; 278(6): H1791 - H1798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Netticadan, R. M. Temsah, K. Kawabata, and N. S. Dhalla Sarcoplasmic Reticulum Ca2+/Calmodulin-Dependent Protein Kinase Is Altered in Heart Failure Circ. Res., March 17, 2000; 86(5): 596 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-I. Kawabata, T. Netticadan, M. Osada, K. Tamura, and N. S. Dhalla Mechanisms of ischemic preconditioning effects on Ca2+ paradox-induced changes in heart Am J Physiol Heart Circ Physiol, March 1, 2000; 278(3): H1008 - H1015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Netticadan, R. Temsah, M. Osada, and N. S. Dhalla Status of Ca2+/calmodulin protein kinase phosphorylation of cardiac SR proteins in ischemia-reperfusion Am J Physiol Cell Physiol, September 1, 1999; 277(3): C384 - C391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Temsah, T. Netticadan, D. Chapman, S. Takeda, S. Mochizuki, and N. S. Dhalla Alterations in sarcoplasmic reticulum function and gene expression in ischemic-reperfused rat heart Am J Physiol Heart Circ Physiol, August 1, 1999; 277(2): H584 - H594. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
