AJP - Heart Calcium Transients and Cell-Sarcomere
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 274: H2123-H2132, 1998;
0363-6135/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gombosová, I.
Right arrow Articles by Neumann, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gombosová, I.
Right arrow Articles by Neumann, J.
Vol. 274, Issue 6, H2123-H2132, June 1998

Postnatal changes in contractile time parameters, calcium regulatory proteins, and phosphatases

Iva Gombosová1, Peter Bokník1, Uwe Kirchhefer1, Jörg Knapp1, Hartmut Lüss1, Frank Ulrich Müller1, Thorsten Müller1, Ute Vahlensieck1, Wilhelm Schmitz1, Geza S. Bodor2, and Joachim Neumann1

1 Institut für Pharmakologie und Toxikologie, Westfälische Wilhelms-Universität, D-48149 Münster, Germany; and 2 Department of Laboratories, Denver Health Medical Center, Denver, Colorado 80204

Compared with isolated electrically driven neonatal ventricular preparations, the total time of contraction, the time to peak tension, and the time of relaxation were decreased to ~50% in adult ventricular preparations. The expression of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) was increased to 133% at the protein level and to 154% at the mRNA level in adult vs. neonatal ventricular preparations, whereas phospholamban was unchanged at both the protein and mRNA levels. Moreover, Ca2+ uptake was increased to 180% in adult vs. neonatal ventricular preparations. Phospholamban phosphorylation was enhanced in adult vs. neonatal ventricular preparations. In adult ventricular preparations, phosphatase activity was reduced to 53% of neonatal preparations, the protein levels of the immunologically detectable catalytic subunits of protein phosphatase types 1 and 2A were reduced to 28 and 61% of neonatal preparations, respectively, and the mRNA levels of type 1alpha , 1beta , 1gamma , 2Aalpha , and 2Abeta phosphatase isoforms were decreased to 69, 68, 54, 67, and 63%, respectively. We conclude that in the adult rat heart, the shortened time parameters of contraction can be explained by an elevated expression of SERCA. In addition, an increased phosphorylation state of phospholamban due to reduced phosphatase activity may be involved.

phospholamban; sarco(endo)plasmic reticulum calcium-adenosinetriphosphatase; calsequestrin; contractility


This article has been cited by other articles:


Home page
 Ann. Thorac. Surg.Home page
J. D. O'Brien, S. E. Howlett, H. J. Burton, S. B. O'Blenes, D. S. Litz, and C. L. H. Friesen
Pediatric cardioplegia strategy results in enhanced calcium metabolism and lower serum troponin T.
Ann. Thorac. Surg., May 1, 2009; 87(5): 1517 - 1523.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. A. Bassani and J. W. M. Bassani
Contribution of Ca2+ transporters to relaxation in intact ventricular myocytes from developing rats
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2406 - H2413.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. Luss, A. Meissner, N. Rolf, H. Van Aken, P. Boknik, U. Kirchhefer, J. Knapp, S. Laer, B. Linck, I. Luss, et al.
Biochemical mechanism(s) of stunning in conscious dogs
Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H176 - H184.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
U. Vahlensieck, P. Bokník, I. Gombosová, S. Huke, J. Knapp, B. Linck, J. Neumann, M. C. Deng, H. H. Scheld, H. Jankowski, et al.
Inotropic Effects of Diadenosine Tetraphosphate (AP4A) in Human and Animal Cardiac Preparations
J. Pharmacol. Exp. Ther., February 1, 1999; 288(2): 805 - 813.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. A. Bassani and J. W. M. Bassani
Contribution of Ca2+ transporters to relaxation in intact ventricular myocytes from developing rats
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2406 - H2413.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online