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Am J Physiol Heart Circ Physiol 274: H2203-H2207, 1998;
0363-6135/98 $5.00
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Vol. 274, Issue 6, H2203-H2207, June 1998

RAPID COMMUNICATION
alpha 1-Adrenergic activation of L-type Ca current in rat ventricular myocytes: perforated patch-clamp recordings

Shi J. Liu1,2 and Richard H. Kennedy2

Departments of 1 Biopharmaceutical Sciences and 2 Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205

alpha 1-Adrenergic stimulation has little effect on L-type Ca2+ channel current (ICa,L) in adult cardiac myocytes measured using conventional whole cell voltage-clamp techniques. In this study using perforated-patch techniques, we reevaluated the effect of alpha 1-adrenergic stimulation on ICa,L in adult rat ventricular myocytes. Action potentials and ICa,L were examined in the presence of 1 µM nadolol, a beta -adrenergic antagonist, in myocytes internally dialyzed with Na+- and K+-free solutions (Cs+ and tetraethylammonium as substitutes). Phenylephrine (PE; 30 µM) increased the action potential duration measured at 25 and 70% of repolarization by 104 and 86%, respectively. In the perforated-patch configuration, PE elicited a transient decrease followed by a ~60% increase in ICa,L, whereas only the transient decrease in ICa,L was observed in myocytes when the conventional whole cell configuration was used. The PE-induced increase in ICa,L was reversibly blocked by 1 µM prazosin, an alpha 1-adrenergic antagonist. These results suggest that alpha 1-adrenergic stimulation enhances cardiac ICa,L and that obligatory intracellular mediators for this action are lost during whole cell recordings.

cardiac muscle cells; whole cell patch clamp; action potential; ion channel


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