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Am J Physiol Heart Circ Physiol 275: H110-H115, 1998;
0363-6135/98 $5.00
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Vol. 275, Issue 1, H110-H115, July 1998

Fluorescent microspheres are superior to radioactive microspheres in chronic blood flow measurements

Matthijs F. M. Van Oosterhout1, Frits W. Prinzen1, S. Sakurada2, Robb W. Glenny3, and J. Robert S. Hales2

1 Department of Physiology, Cardiovascular Research Institute Maastricht, University of Maastricht, 6200 MD Maastricht, The Netherlands; 2 School of Physiology and Pharmacology of the University of New South Wales, Sydney, New South Wales 2260, Australia; and 3 Departments of Medicine and of Physiology and Biophysics, University of Washington, Seattle, Washington 98195

The accuracy of the fluorescent (FM) and radioactive microsphere (RM) techniques is similar in acute experiments but has not been established in chronic experiments. In the present study various combinations (at least pairs) of FM and/or RM labels were injected simultaneously between 2 mo and 5 min before each animal was killed. Blood flow was determined in many organs. Intramethod mean difference and variation did not change over time for FM but increased significantly for RM (from 1.8 ± 1.4 to 25.6 ± 21.8% and from 4.4 ± 3.2 to 32.4 ± 23.0% at 5 min and 2 mo, respectively). Also the FM-RM intermethod mean difference and variation increased (from -0.5 ± 8.5 to 40.8 ± 23.8% and from 23.6 ± 4.6 to 71.8 ± 34.3%, respectively). After 2 mo, blood flow estimations were 20-50% lower with the various RM, whereas brain and liver blood flow values varied even more between isotopes. Underestimation started within 1 day for 51Cr and within 2 wk for 141Ce, 95Nb, and 85Sr. We conclude that FM are superior to RM for blood flow determination in experiments lasting longer than 1 day, presumably because of leaching of isotopes from RM.

nonradioactive microspheres; fluorescence; organ flow; chronic animal experiments; cardiac output distribution


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