AJP - Heart AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 275: H416-H421, 1998;
0363-6135/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gunnett, C. A.
Right arrow Articles by Faraci, F. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gunnett, C. A.
Right arrow Articles by Faraci, F. M.
Vol. 275, Issue 2, H416-H421, August 1998

Vascular effects of LPS in mice deficient in expression of the gene for inducible nitric oxide synthase

Carol A. Gunnett, Yi Chu, Donald D. Heistad, Angela Loihl, and Frank M. Faraci

Departments of Internal Medicine and Pharmacology, Cardiovascular Center, University of Iowa College of Medicine, Iowa City, Iowa 52242

The inducible isoform of nitric oxide synthase (iNOS) is expressed after systemic administration of lipopolysaccharide (LPS). The importance of expression of iNOS in blood vessels is poorly defined. Because nitric oxide from iNOS may alter vasomotor function, we examined effects of LPS on vasomotor function in carotid arteries from iNOS-deficient mice. We studied contraction of the carotid artery from wild-type and iNOS-deficient mice in vitro 12 h after injection of LPS (20 mg/kg ip). Contractile responses to PGF2alpha (3-30 µM) and thromboxane A2 analog (U-46619; 3-100 nM) were evaluated using vascular rings from mice treated with vehicle or LPS. Maximum force of contraction generated by rings in response to PGF2alpha was 0.39 ± 0.02 and 0.25 ± 0.01 (SE) g (n = 14) in vehicle and LPS-treated wild-type mice, respectively (P < 0.001 vs. vehicle). Thus LPS reduced constrictor responses in wild-type mice. Thiocitrulline and aminoguanidine (inhibitors of iNOS) improved contractile responses from LPS-treated wild-type vessels. Indomethacin also improved constrictor responses in arteries from wild-type mice injected with LPS. In contrast, contraction of the carotid arteries in response to PGF2alpha and U-46619 was not impaired in LPS-treated iNOS-deficient mice, and contraction was not altered by inhibitors of iNOS. Expression of iNOS mRNA was confirmed using RT-PCR in carotid arteries from wild-type mice after injection of LPS but not vehicle. PCR products for iNOS were not observed in iNOS-deficient mice. These findings provide the first direct evidence that iNOS mediates impairment of vascular contraction after treatment with LPS.

carotid artery; acetylcholine; vasoconstriction; reverse transcriptase-polymerase chain reaction; lipopolysaccharide


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. D. Lund, R. M. Brooks, F. M. Faraci, and D. D. Heistad
Role of angiotensin II in endothelial dysfunction induced by lipopolysaccharide in mice
Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3726 - H3731.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. D. Lund, Y. Chu, R. M. Brooks, F. M. Faraci, and D. D. Heistad
Effects of a common human gene variant of extracellular superoxide dismutase on endothelial function after endotoxin in mice
J. Physiol., October 15, 2007; 584(2): 583 - 590.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. M. Tiwari, R. W. Brock, J. K. Megyesi, G. P. Kaushal, and P. R. Mayeux
Disruption of renal peritubular blood flow in lipopolysaccharide-induced renal failure: role of nitric oxide and caspases
Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1324 - F1332.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. A. Gunnett, D. D. Lund, F. M. Faraci, and D. D. Heistad
Vascular interleukin-10 protects against LPS-induced vasomotor dysfunction
Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H624 - H630.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. K. Wilkerson, L. A. Lesniewski, E. M. Golding, R. M. Bryan Jr., A. Amin, E. Wilson, and M. D. Delp
Simulated microgravity enhances cerebral artery vasoconstriction and vascular resistance through endothelial nitric oxide mechanism
Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1652 - H1661.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. D. Lund, C. A. Gunnett, Y. Chu, R. M. Brooks, F. M. Faraci, and D. D. Heistad
Gene transfer of extracellular superoxide dismutase improves relaxation of aorta after treatment with endotoxin
Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H805 - H811.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Eguchi, L. V. d'Uscio, C. Wambi, D. Weiler, I. Kovesdi, T. O'Brien, and Z. S. Katusic
Inhibitory effect of recombinant iNOS gene expression on vasomotor function of canine basilar artery
Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2560 - H2566.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. Sugita, M. Kaneki, E. Tokunaga, M. Sugita, C. Koike, S. Yasuhara, R. G. Tompkins, and J. A. J. Martyn
Inducible nitric oxide synthase plays a role in LPS-induced hyperglycemia and insulin resistance
Am J Physiol Endocrinol Metab, February 1, 2002; 282(2): E386 - E394.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. Tyml, X. Wang, D. Lidington, and Y. Ouellette
Lipopolysaccharide reduces intercellular coupling in vitro and arteriolar conducted response in vivo
Am J Physiol Heart Circ Physiol, September 1, 2001; 281(3): H1397 - H1406.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Chrissobolis, J. Ziogas, Y. Chu, F. M. Faraci, and C. G. Sobey
Role of inwardly rectifying K+ channels in K+-induced cerebral vasodilatation in vivo
Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H2704 - H2712.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. A. Gunnett, D. D. Heistad, A. Loihl, and F. M. Faraci
Tumor necrosis factor-alpha impairs contraction but not relaxation in carotid arteries from iNOS-deficient mice
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2000; 279(5): R1558 - R1564.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. A. Gunnett, D. D. Heistad, D. J. Berg, and F. M. Faraci
IL-10 deficiency increases superoxide and endothelial dysfunction during inflammation
Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1555 - H1562.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J.-L. VINCENT, H. ZHANG, C. SZABO, and J.-C. PREISER
Effects of Nitric Oxide in Septic Shock
Am. J. Respir. Crit. Care Med., June 1, 2000; 161(6): 1781 - 1785.
[Abstract] [Full Text]

Home page
 StrokeHome page
S. P. Didion, C. D. Sigmund, F. M. Faraci, and Z. S. Katusic
Impaired Endothelial Function in Transgenic Mice Expressing Both Human Renin and Human Angiotensinogen • Editorial Comment
Stroke, March 1, 2000; 31(3): 760 - 765.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
F. M. Faraci and C. D. Sigmund
Vascular Biology in Genetically Altered Mice : Smaller Vessels, Bigger Insight
Circ. Res., December 3, 1999; 85(12): 1214 - 1225.
[Full Text] [PDF]

Home page
 StrokeHome page
C. A. Gunnett, D. J. Berg, F. M. Faraci, and G. Feuerstein
Vascular Effects of Lipopolysaccharide Are Enhanced in Interleukin-10-Deficient Mice • Editorial Comment
Stroke, October 1, 1999; 30(10): 2191 - 2196.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online