| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Cardiovascular Research Group, Departments of 1 Pharmacology and 2 Pediatrics, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Glycogen and its
turnover are important components of myocardial glucose metabolism that
significantly impact on postischemic recovery. We developed a method to
measure glycogen turnover (rates of glycogen synthesis and degradation)
in isolated working rat hearts using
[3H]- and
[14C]glucose. In
aerobic hearts perfused with 11 mM glucose, 1.2 mM palmitate, and 100 µU/ml insulin, rates of glycogen synthesis and degradation were 1.24 ± 0.3 and 0.53 ± 0.25 µmol · min
1 · g
dry wt1, respectively.
Low-flow ischemia (0.5 ml/min, 60 min) elicited a marked
glycogenolysis; rates of glycogen synthesis and degradation were 0.54 ± 0.16 and 2.12 ± 0.14 µmol · min1 · g
dry wt1, respectively.
During reperfusion (30 min), mechanical function recovered to 20% of
preischemic values. Rates of synthesis and degradation were 1.66 ± 0.16 and 1.55 ± 0.21 µmol · min1 · g
dry wt1, respectively, and
glycogen content remained unchanged (25 ± 3 µmol/g dry wt). The
assessment of glycogen metabolism needs to take into account the
simultaneous synthesis and degradation of glycogen. With this approach,
a substantial turnover of glycogen was detectable not only during
aerobic conditions but also during ischemia as well as reperfusion.
myocardial energy metabolism; left ventricular work; reperfusion injury; glucose oxidation; glycolysis; proton production
This article has been cited by other articles:
|
|
 |
|
  M. Gandhi, B. A. Finegan, and A. S. Clanachan Role of glucose metabolism in the recovery of postischemic LV mechanical function: effects of insulin and other metabolic modulators Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2576 - H2586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Omar, H. Fraser, and A. S. Clanachan Ischemia-induced activation of AMPK does not increase glucose uptake in glycogen-replete isolated working rat hearts Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1266 - H1273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Belke, E. Swanson, J. Suarez, B. T. Scott, A. E. Stenbit, and W. H. Dillmann Increased expression of SERCA in the hearts of transgenic mice results in increased oxidation of glucose Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1755 - H1763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Jaswal, M. Gandhi, B. A. Finegan, J. R. B. Dyck, and A. S. Clanachan Effects of adenosine on myocardial glucose and palmitate metabolism after transient ischemia: role of 5'-AMP-activated protein kinase Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1883 - H1892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Tracey, J. L. Treadway, W. P. Magee, J. C. Sutt, R. K. McPherson, C. B. Levy, D. E. Wilder, L. J. Yu, Y. Chen, R. M. Shanker, et al. Cardioprotective effects of ingliforib, a novel glycogen phosphorylase inhibitor Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H1177 - H1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Griffin, L. T. White, and E. D. Lewandowski Substrate-dependent proton load and recovery of stunned hearts during pyruvate dehydrogenase stimulation Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H361 - H367. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
