AJP - Heart AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 275: H1533-H1541, 1998;
0363-6135/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fraser, H.
Right arrow Articles by Clanachan, A. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fraser, H.
Right arrow Articles by Clanachan, A. S.
Vol. 275, Issue 5, H1533-H1541, November 1998

Assessment of glycogen turnover in aerobic, ischemic, and reperfused working rat hearts

Heather Fraser1, Gary D. Lopaschuk1,2, and Alexander S. Clanachan1

Cardiovascular Research Group, Departments of 1 Pharmacology and 2 Pediatrics, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada T6G 2H7

Glycogen and its turnover are important components of myocardial glucose metabolism that significantly impact on postischemic recovery. We developed a method to measure glycogen turnover (rates of glycogen synthesis and degradation) in isolated working rat hearts using [3H]- and [14C]glucose. In aerobic hearts perfused with 11 mM glucose, 1.2 mM palmitate, and 100 µU/ml insulin, rates of glycogen synthesis and degradation were 1.24 ± 0.3 and 0.53 ± 0.25 µmol · min-1 · g dry wt-1, respectively. Low-flow ischemia (0.5 ml/min, 60 min) elicited a marked glycogenolysis; rates of glycogen synthesis and degradation were 0.54 ± 0.16 and 2.12 ± 0.14 µmol · min-1 · g dry wt-1, respectively. During reperfusion (30 min), mechanical function recovered to 20% of preischemic values. Rates of synthesis and degradation were 1.66 ± 0.16 and 1.55 ± 0.21 µmol · min-1 · g dry wt-1, respectively, and glycogen content remained unchanged (25 ± 3 µmol/g dry wt). The assessment of glycogen metabolism needs to take into account the simultaneous synthesis and degradation of glycogen. With this approach, a substantial turnover of glycogen was detectable not only during aerobic conditions but also during ischemia as well as reperfusion.

myocardial energy metabolism; left ventricular work; reperfusion injury; glucose oxidation; glycolysis; proton production


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Gandhi, B. A. Finegan, and A. S. Clanachan
Role of glucose metabolism in the recovery of postischemic LV mechanical function: effects of insulin and other metabolic modulators
Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2576 - H2586.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. A. Omar, H. Fraser, and A. S. Clanachan
Ischemia-induced activation of AMPK does not increase glucose uptake in glycogen-replete isolated working rat hearts
Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1266 - H1273.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. D. Belke, E. Swanson, J. Suarez, B. T. Scott, A. E. Stenbit, and W. H. Dillmann
Increased expression of SERCA in the hearts of transgenic mice results in increased oxidation of glucose
Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1755 - H1763.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. S. Jaswal, M. Gandhi, B. A. Finegan, J. R. B. Dyck, and A. S. Clanachan
Effects of adenosine on myocardial glucose and palmitate metabolism after transient ischemia: role of 5'-AMP-activated protein kinase
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1883 - H1892.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. R. Tracey, J. L. Treadway, W. P. Magee, J. C. Sutt, R. K. McPherson, C. B. Levy, D. E. Wilder, L. J. Yu, Y. Chen, R. M. Shanker, et al.
Cardioprotective effects of ingliforib, a novel glycogen phosphorylase inhibitor
Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H1177 - H1184.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. L. Griffin, L. T. White, and E. D. Lewandowski
Substrate-dependent proton load and recovery of stunned hearts during pyruvate dehydrogenase stimulation
Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H361 - H367.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online