AJP - Heart pressure measurements
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 275: H1652-H1662, 1998;
0363-6135/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tóth, A.
Right arrow Articles by Ligeti, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tóth, A.
Right arrow Articles by Ligeti, L.
Vol. 275, Issue 5, H1652-H1662, November 1998

Quantitative assessment of [Ca2+]i levels in rat skeletal muscle in vivo

András Tóth1, Tamás Ivanics1, Zoltán Ruttner1, Dick W. Slaaf2, Robert S. Reneman3, and László Ligeti1

1 Second Department of Physiology, Semmelweis Medical University, H-1082 Budapest, Hungary; and Departments of 2 Biophysics and 3 Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands

Intracellular free Ca2+ concentration ([Ca2+]i) plays an essential role in physiological regulatory processes and common pathological conditions. Better understanding of these phenomena is still hampered by problems encountered in the quantitative assessment of [Ca2+]i changes, especially in blood-perfused organs. This study demonstrates that the ratiometric fluorescence technique can be adapted for quantitative in vivo [Ca2+]i determinations. The rat spinotrapezius muscle was topically loaded with indo 1-AM and imaged by a cooled digital camera. Ratio images were calculated in small regions (100 µm × 100 µm) practically devoid of large vessels in the resting state, after 30 min of ischemia, 20 min of reperfusion, or ionomycin or manganate treatments. When we assumed an average [Ca2+]i of 100 nM in the resting blood-perfused muscle, ischemia increased [Ca2+]i to ~200 nM. During reperfusion [Ca2+]i decreased to ~140 nM. Ionomycin induced an increase in [Ca2+]i to well above 750 nM. Manganate reduced Ca2+-dependent fluorescence to virtually zero. Our main conclusion is that changes in [Ca2+]i can be monitored and quantitatively determined in vivo.

intravital microscopy; indo 1-acetoxymethyl ester ratiometric method; ischemia-reperfusion; ionomycin


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. Sonobe, T. Inagaki, D. C. Poole, and Y. Kano
Intracellular calcium accumulation following eccentric contractions in rat skeletal muscle in vivo: role of stretch-activated channels
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2008; 294(4): R1329 - R1337.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Terada, I. Muraoka, and I. Tabata
Changes in [Ca2+]i induced by several glucose transport-enhancing stimuli in rat epitrochlearis muscle
J Appl Physiol, May 1, 2003; 94(5): 1813 - 1820.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. K. Bailey, C. A. Kindig, B. J. Behnke, T. I. Musch, G. W. Schmid-Schoenbein, and D. C. Poole
Spinotrapezius muscle microcirculatory function: effects of surgical exteriorization
Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H3131 - H3137.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online