AJP - Heart Calcium Transients and Cell-Sarcomere
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 275: H1845-H1856, 1998;
0363-6135/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vagnoni, K. E.
Right arrow Articles by Magness, R. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vagnoni, K. E.
Right arrow Articles by Magness, R. R.
Vol. 275, Issue 5, H1845-H1856, November 1998

Endothelial vasodilator production by uterine and systemic arteries. III. Ovarian and estrogen effects on NO synthase

Karen E. Vagnoni1, Cynthia E. Shaw1, Terrance M. Phernetton1, Beth M. Meglin1, Ian M. Bird1, and Ronald R. Magness1,2

1 Perinatal Research Laboratories, Department of Obstetrics and Gynecology, and 2 Department of Meat and Animal Science, University of Wisconsin-Madison Medical School, Madison, Wisconsin 53715

During the follicular phase of the ovarian cycle, when the local estrogen-to-progesterone ratio is elevated, uterine blood flow is elevated. This vasodilatory response is reproduced by exogenous 17beta -estradiol (E2beta ) administration via a nitric oxide (NO)-mediated mechanism. We hypothesized that endogenous ovarian estrogen and exogenous E2beta treatment elevate expression of endothelial cell-derived NO synthase (eNOS) in uterine, but not in systemic, arteries. Uterine, mammary, and systemic (renal and/or omental) arteries were collected from 1) ewes synchronized to the follicular (day -1 to day 0) or luteal (day 10) phases of the ovarian cycle (n = 4 per phase), 2) ovariectomized ewes 120 min after systemic vehicle or E2beta (5 µg/kg iv) treatment, and 3) ovariectomized ewes on days 0, 3, 6, 8, and 10 of E2beta (5 µg/kg iv, followed by 6 µg/kg per day) treatment. Expression of eNOS was localized primarily to the endothelium rather than vascular smooth muscle (VSM) in all arteries examined by immunohistochemistry and Western analysis; inducible NOS was not detected in either endothelium or VSM. Expression of eNOS protein was greater (P < 0.05) in uterine, but not in systemic, artery endothelium-isolated protein collected from follicular versus luteal phase ewes. Acute systemic E2beta treatment of ovariectomized ewes increased (P < 0.05) eNOS protein levels in uterine artery endothelium. Prolonged E2beta administration progressively increased uterine, but not systemic, artery endothelial eNOS protein expression. Therefore, the increased local estrogen-to-progesterone ratio during the follicular phase locally elevates eNOS expression, which possibly elevates uterine blood flow. These responses can be partly reproduced with E2beta administration.

ovary; uterine blood flow; steroids; nitric oxide; mammary; renal


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P.-A. Scott, A. Tremblay, M. Brochu, and J. St-Louis
Vasorelaxant action of 17 -estradiol in rat uterine arteries: role of nitric oxide synthases and estrogen receptors
Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3713 - H3719.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. E. Sweeney, P. Bagher, J. Bailey, S. J. Cherra 3rd, F. N. Grisafi, E. M. Pauli, K. Riley, and S. Soares
Intravascular pressure and diameter profile of the utero-ovarian resistance artery network: estrous cycle-dependent modulation of resistance artery tone
Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2937 - H2944.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
F. Li, C. E. Wood, and M. Keller-Wood
Adrenalectomy alters regulation of blood pressure and endothelial nitric oxide synthase in sheep: modulation by estradiol
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R257 - R266.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Kawai, Y. Yokoyama, S. Kawai, S. Yokoyama, K. Oda, T. Nagasaka, M. Nagino, I. H. Chaudry, and Y. Nimura
Does estrogen contribute to the hepatic regeneration following portal branch ligation in rats?
Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G582 - G589.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Nagar, X.-t. Liu, and C. R. Rosenfeld
Estrogen regulates {beta}1-subunit expression in Ca2+-activated K+ channels in arteries from reproductive tissues
Am J Physiol Heart Circ Physiol, October 1, 2005; 289(4): H1417 - H1427.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
F.-X. Yi, R. R. Magness, and I. M. Bird
Simultaneous imaging of [Ca2+]i and intracellular NO production in freshly isolated uterine artery endothelial cells: effects of ovarian cycle and pregnancy
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R140 - R148.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. B. Suliman, M. Ali, and C. A. Piantadosi
Superoxide dismutase-3 promotes full expression of the EPO response to hypoxia
Blood, July 1, 2004; 104(1): 43 - 50.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. M. Muller-Delp, D. B. Lubahn, K. E. Nichol, B. J. Philips, E. M. Price, E. M. Curran, and M. H. Laughlin
Regulation of nitric oxide-dependent vasodilation in coronary arteries of estrogen receptor-{alpha}-deficient mice
Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H2150 - H2157.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. H. Laughlin, W. V. Welshons, M. Sturek, J. W. E. Rush, J. R. Turk, J. A. Taylor, B. M. Judy, K. K. Henderson, and V. K. Ganjam
Gender, exercise training, and eNOS expression in porcine skeletal muscle arteries
J Appl Physiol, July 1, 2003; 95(1): 250 - 264.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
I. M. Bird, L. Zhang, and R. R. Magness
Possible mechanisms underlying pregnancy-induced changes in uterine artery endothelial function
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2003; 284(2): R245 - R258.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. R. Bell, K. E. Gochenaur, and J. Hecht
O2--mediated impairment of coronary arterial relaxation is prevented by overnight treatment with 1 nM beta -estradiol
J Appl Physiol, December 1, 2002; 93(6): 1952 - 1958.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. L. Mershon, R. S. Baker, and K. E. Clark
Estrogen increases iNOS expression in the ovine coronary artery
Am J Physiol Heart Circ Physiol, September 1, 2002; 283(3): H1169 - H1180.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. Mukundan, T. C. Resta, and N. L. Kanagy
17beta -Estradiol decreases hypoxic induction of erythropoietin gene expression
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2002; 283(2): R496 - R504.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. L. Rupnow, T. M. Phernetton, M. L. Modrick, M. C. Wiltbank, I. M. Bird, and R. R. Magness
Endothelial Vasodilator Production by Uterine and Systemic Arteries. VIII. Estrogen and Progesterone Effects on cPLA2, COX-1, and PGIS Protein Expression
Biol Reprod, February 1, 2002; 66(2): 468 - 474.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. M. Joyce, T. M. Phernetton, C. E. Shaw, M. L. Modrick, and R. R. Magness
Endothelial vasodilator production by uterine and systemic arteries. IX. eNOS gradients in cycling and pregnant ewes
Am J Physiol Heart Circ Physiol, January 1, 2002; 282(1): H342 - H348.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. R. Rosenfeld
Mechanisms regulating angiotensin II responsiveness by the uteroplacental circulation
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2001; 281(4): R1025 - R1040.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. R. Rosenfeld, D. N. Cornfield, and T. Roy
Ca2+-activated K+ channels modulate basal and E2{beta}-induced rises in uterine blood flow in ovine pregnancy
Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H422 - H431.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. R. Magness, J. A. Sullivan, Y. Li, T. M. Phernetton, and I. M. Bird
Endothelial vasodilator production by uterine and systemic arteries. VI. Ovarian and pregnancy effects on eNOS and NOx
Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1692 - H1698.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. L. Rupnow, T. M. Phernetton, C. E. Shaw, M. L. Modrick, I. M. Bird, and R. R. Magness
Endothelial vasodilator production by uterine and systemic arteries. VII. Estrogen and progesterone effects on eNOS
Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1699 - H1705.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Zhang, K. G. Stewart, and S. T. Davidge
Endogenous estrogen mediates vascular reactivity and distensibility in pregnant rat mesenteric arteries
Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H956 - H961.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. H. Laughlin, W. G. Schrage, R. M. McAllister, H. A. Garverick, and A. W. Jones
Interaction of gender and exercise training: vasomotor reactivity of porcine skeletal muscle arteries
J Appl Physiol, January 1, 2001; 90(1): 216 - 227.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
L. P. Thompson, G. Pinkas, and C. P. Weiner
Chronic 17{beta}-Estradiol Replacement Increases Nitric Oxide-Mediated Vasodilation of Guinea Pig Coronary Microcirculation
Circulation, July 25, 2000; 102(4): 445 - 451.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. R. Rosenfeld, R. E. White, T. Roy, and B. E. Cox
Calcium-activated potassium channels and nitric oxide coregulate estrogen-induced vasodilation
Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H319 - H328.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. A. Salhab, P. W. Shaul, B. E. Cox, and C. R. Rosenfeld
Regulation of types I and III NOS in ovine uterine arteries by daily and acute estrogen exposure
Am J Physiol Heart Circ Physiol, June 1, 2000; 278(6): H2134 - H2142.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
I. A. Buhimschi, C. Yallampalli, C. S. Buhimschi, G. R. Saade, and R. E. Garfield
Distinct regulation of nitric oxide and cyclic guanosine monophosphate production by steroid hormones in the rat uterus
Mol. Hum. Reprod., May 1, 2000; 6(5): 404 - 414.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
P.R.R. Gangula, S.J. Wimalawansa, and C. Yallampalli
Pregnancy and sex steroid hormones enhance circulating calcitonin gene-related peptide concentrations in rats
Hum. Reprod., April 1, 2000; 15(4): 949 - 953.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online