| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Internal Medicine, Pulmonary and Critical Care Division, and Department of Physiology, University of Missouri-Columbia, Columbia, Missouri 65212; and 2 Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130
Nitric oxide
synthase (NOS) inhibitors have been reported to increase as well as to
decrease microvascular transport of macromolecules in a variety of
models. This study was performed to determine whether the influence of
NOS inhibition on albumin leakage was dependent on the fluorescent dyes
used to label albumin. Albumin leakage was assessed in rat mesenteric
venules during control conditions and after exposure to the NOS
inhibitor NG-nitro-L-arginine methyl
ester (L-NAME). Albumin was
labeled with any one of four dyes: FITC, sulforhodamine 101 [Texas Red (TR)], dichlorotriazinyl aminofluorescein
(DTAF), or Oregon Green 514 (OG). Superfusion with
L-NAME
(10
4 M) was accompanied by
an increase in leakage of FITC-labeled albumin
(n = 12) but not of albumin labeled
with DTAF (n = 10), TR
(n = 10), or OG
(n = 4). In vessels perfused with both
FITC- and TR-labeled albumin (n = 12),
superfusion with L-NAME
increased leakage of FITC- but not TR-labeled albumin. In conclusion,
albumin leakage responses to
L-NAME differ among various
fluorescent dyes. Therefore, caution is advised in comparison of
albumin leakage results that utilize different fluorescent dyes.
macromolecular leakage; nitric oxide; photohemolysis
This article has been cited by other articles:
|
|
 |
|
  M.-H. Kim, F.-R. E. Curry, and S. I. Simon Dynamics of neutrophil extravasation and vascular permeability are uncoupled during aseptic cutaneous wounding Am J Physiol Cell Physiol, April 1, 2009; 296(4): C848 - C856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Burns, Z. Zheng, S. H. Soubra, J. Chen, and R. E. Rumbaut Transendothelial flow inhibits neutrophil transmigration through a nitric oxide-dependent mechanism: potential role for cleft shear stress Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2904 - H2910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. H. Huxley, J. J. Wang, and I. H. Sarelius Adaptation of coronary microvascular exchange in arterioles and venules to exercise training and a role for sex in determining permeability responses Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1196 - H1205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. H. Huxley, J. Wang, and S. P. Whitt Sexual dimorphism in the permeability response of coronary microvessels to adenosine Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H2006 - H2013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Flessner The transport barrier in intraperitoneal therapy Am J Physiol Renal Physiol, March 1, 2005; 288(3): F433 - F442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. O. Dull, B. J. DeWitt, R. Dinavahi, L. Schwartz, C. Hubert, N. Pace, and C. Fronticelli Quantitative assessment of hemoglobin-induced endothelial barrier dysfunction J Appl Physiol, November 1, 2004; 97(5): 1930 - 1937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zeng, H. Zhang, C. Lowell, and P. He Tumor necrosis factor-alpha -induced leukocyte adhesion and microvessel permeability Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2420 - H2430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Rumbaut, J. Wang, and V. H. Huxley Differential effects of L-NAME on rat venular hydraulic conductivity Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H2017 - H2023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Baldwin Modified hemoglobins produce venular interendothelial gaps and albumin leakage in the rat mesentery Am J Physiol Heart Circ Physiol, August 1, 1999; 277(2): H650 - H659. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
