AJP - Heart AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 276: H503-H508, 1999;
0363-6135/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lombard, J. H.
Right arrow Articles by Jackson, W. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lombard, J. H.
Right arrow Articles by Jackson, W. F.
Vol. 276, Issue 2, H503-H508, February 1999

Cytochrome P-450 omega -hydroxylase senses O2 in hamster muscle, but not cheek pouch epithelium, microcirculation

Julian H. Lombard1, Mary Pat Kunert2, Richard J. Roman1, John R. Falck3, David R. Harder1, and William F. Jackson4

1 Department of Physiology, Medical College of Wisconsin, Milwaukee 53226; 2 College of Nursing, Marquette University, Milwaukee, Wisconsin 53202; 3 Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75235; and 4 Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008

The goal of this study was to investigate the role of cytochrome P-450 omega -hydroxylase in mediating O2-induced constriction of arterioles in the microcirculation of the hamster. Male Golden hamsters were anesthetized with pentobarbital sodium, and the cremaster muscle or cheek pouch was prepared for observation by intravital microscopy. Arteriolar diameters were measured during elevations of superfusate PO2 from ~5 to 150 mmHg. Arteriolar responses to elevated PO2 were determined in the cremaster muscle, in the retractor muscle where it inserts on the cheek pouch, and in the epithelial portion of the cheek pouch. Elevation of superfusion solution PO2 caused a vigorous constriction of arterioles in the cremaster and retractor muscles and in the epithelial portion of the cheek pouch. Superfusion with 10 µM 17-octadecynoic acid, a suicide substrate inhibitor of cytochrome P-450 omega -hydroxylase, and intravenous infusion of N-methylsulfonyl-12,12-dibromododec-11-enamide, a mechanistically different and highly selective inhibitor of cytochrome P-450 omega -hydroxylase, caused a significant reduction in the magnitude of O2-induced constriction of arterioles in the cremaster and retractor muscles. However, arteriolar constriction in response to elevated PO2 was unaffected by 17-octadecynoic acid or N-methylsulfonyl-12,12-dibromododec-11-enamide in the epithelial portion of the cheek pouch. These data confirm that there are regional differences in the mechanism of action of O2 on the microcirculation and indicate that cytochrome P-450 omega -hydroxylase senses O2 in the microcirculation of hamster skeletal muscle, but not in the cheek pouch epithelium.

arterioles; oxygen; 20-hydroxyeicosatetraenoic acid; 17-octadecynoic acid; vasoconstriction


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. J. Marvar, J. R. Falck, and M. A. Boegehold
High dietary salt reduces the contribution of 20-HETE to arteriolar oxygen responsiveness in skeletal muscle
Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1507 - H1515.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Wang, R. J. Roman, J. R. Falck, L. de la Cruz, and J. H. Lombard
Effects of high-salt diet on CYP450-4A {omega}-hydroxylase expression and active tone in mesenteric resistance arteries
Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1557 - H1565.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Fuloria, D. M. Eckman, D. A. Leach, and J. L. Aschner
20-Hydroxyeicosatetraenoic acid is a vasoconstrictor in the newborn piglet pulmonary microcirculation
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L360 - L365.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. C. Frisbee, K. G. Maier, J. R. Falck, R. J. Roman, and J. H. Lombard
Integration of hypoxic dilation signaling pathways for skeletal muscle resistance arteries
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2002; 283(2): R309 - R319.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
R. J. Roman
P-450 Metabolites of Arachidonic Acid in the Control of Cardiovascular Function
Physiol Rev, January 1, 2002; 82(1): 131 - 185.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. Platoshyn, Y. Yu, V. A. Golovina, S. S. McDaniel, S. Krick, L. Li, J.-Y. Wang, Lewis. J. Rubin, and J. X.-J. Yuan
Chronic hypoxia decreases KV channel expression and function in pulmonary artery myocytes
Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L801 - L812.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. P. Kunert, R. J. Roman, M. Alonso-Galicia, J. R. Falck, and J. H. Lombard
Cytochrome P-450 {omega}-hydroxylase: a potential O2 sensor in rat arterioles and skeletal muscle cells
Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1840 - H1845.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. G. Welsh and S. S. Segal
Role of EDHF in conduction of vasodilation along hamster cheek pouch arterioles in vivo
Am J Physiol Heart Circ Physiol, June 1, 2000; 278(6): H1832 - H1839.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. C. Frisbee, J. R. Falck, and J. H. Lombard
Contribution of cytochrome P-450 omega -hydroxylase to altered arteriolar reactivity with high-salt diet and hypertension
Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1517 - H1526.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. E. HUNGERFORD, W. C. SESSA, and S. S. SEGAL
Vasomotor control in arterioles of the mouse cremaster muscle
FASEB J, January 1, 2000; 14(1): 197 - 207.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
B. FLEMMING, E. SEELIGER, T. WRONSKI, K. STEER, N. ARENZ, and P. B. PERSSON
Oxygen and Renal Hemodynamics in the Conscious Rat
J. Am. Soc. Nephrol., January 1, 2000; 11(1): 18 - 24.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online