AJP - Heart BIOPAC complete lab solutions
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 276: H1207-H1214, 1999;
0363-6135/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Terraz, S.
Right arrow Articles by Feihl, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Terraz, S.
Right arrow Articles by Feihl, F.
Vol. 276, Issue 4, H1207-H1214, April 1999

Hypoxic contraction of small pulmonary arteries from normal and endotoxemic rats: fundamental role of NO

Sylvain Terraz1, François Baechtold1, Delphine Renard1, Attila Barsi1, Anne Rosselet1, Alex Gnaegi1, Lucas Liaudet1, Romain Lazor1, Jacques-A. Haefliger2, Nicolas Schaad5, Claude Perret1, Paul Kucera4, Michèle Markert3, and Francois Feihl1

1 Division of Clinical Pathophysiology and 2 Laboratory of Molecular Biology, Department of Internal Medicine, and 3 Central Laboratory of Clinical Chemistry, Lausanne University Hospital, 1011 Lausanne; 4 Institute of Physiology, Lausanne University Medical School, 1005 Lausanne; and 5 Pharmacie Interhospitalière de La Côte, 1110 Morges, Switzerland

The present study was aimed at examining the role of nitric oxide (NO) in the hypoxic contraction of isolated small pulmonary arteries (SPA) in the rat. Animals were treated with either saline (sham experiments) or Escherichia coli lipolysaccharide [LPS, to obtain expression of the inducible NO synthase (iNOS) in the lung] and killed 4 h later. SPA (300- to 600-µm outer diameter) were mounted as rings in organ chambers for the recording of isometric tension, precontracted with PGF2alpha , and exposed to either severe (bath PO2 8 ± 3 mmHg) or milder (21 ± 3 mmHg) hypoxia. In SPA from sham-treated rats, contractions elicited by severe hypoxia were completely suppressed by either endothelium removal or preincubation with an NOS inhibitor [NG-nitro-L-arginine methyl ester (L-NAME), 10-3 M]. In SPA from LPS-treated rats, contractions elicited by severe hypoxia occurred irrespective of the presence or absence of endothelium and were largely suppressed by L-NAME. The milder hypoxia elicited no increase in vascular tone. These results indicate an essential role of NO in the hypoxic contractions of precontracted rat SPA. The endothelium independence of HPV in arteries from LPS-treated animals appears related to the extraendothelial expression of iNOS. The severe degree of hypoxia required to elicit any contraction is consistent with a mechanism of reduced NO production caused by a limited availability of O2 as a substrate for NOS.

pulmonary circulation; hypoxia; vascular endothelium; nitric oxide; endotoxin; rat


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Olson, R. Oeckler, X. Li, L. Du, F. Traganos, X. Zhao, and T. Burke-Wolin
Angiotensin II stimulates nitric oxide production in pulmonary artery endothelium via the type 2 receptor
Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L559 - L568.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
E. Nozik-Grayck, Y.-C. T. Huang, M. S. Carraway, and C. A. Piantadosi
Bicarbonate-dependent superoxide release and pulmonary artery tone
Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2327 - H2335.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. G. Davidson, A. D. Bersten, H. A. Barr, K. D. Dowling, T. E. Nicholas, and I. R. Doyle
Endotoxin Induces Respiratory Failure and Increases Surfactant Turnover and Respiration Independent of Alveolocapillary Injury in Rats
Am. J. Respir. Crit. Care Med., June 1, 2002; 165(11): 1516 - 1525.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. D. Le Cras and I. F. McMurtry
Nitric oxide production in the hypoxic lung
Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L575 - L582.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. TSUCHIYA, H. TOKAI, Y. TAKEHARA, Y. HARAGUCHI, A. ASADA, K. UTSUMI, and M. INOUE
Interrelation between Oxygen Tension and Nitric Oxide in the Respiratory System
Am. J. Respir. Crit. Care Med., October 1, 2000; 162(4): 1257 - 1261.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online