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Am J Physiol Heart Circ Physiol 276: H1236-H1244, 1999;
0363-6135/99 $5.00
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Vol. 276, Issue 4, H1236-H1244, April 1999

Osmotic shock: modulation of contractile function, pHi, and ischemic damage in perfused guinea pig heart

Douglas E. Befroy1, Trevor Powell2, George K. Radda1, and Kieran Clarke1

1 Department of Biochemistry and 2 University Laboratory of Physiology, University of Oxford, Oxford OX1 3QU, United Kingdom

To determine the contribution of changes in extracellular osmolarity to ischemic injury, isolated guinea pig hearts were perfused with hyposmotic (220 mosM) or hyperosmotic (380 mosM) buffer. 31P NMR spectroscopy was used to follow changes in intracellular pH (pHi) and energetics. Hyposmotic buffer decreased myocardial developed pressure by 30 ± 2% and pHi by 0.02 ± 0.01 unit, whereas hyperosmotic buffer increased myocardial developed pressure by 34 ± 1% and pHi by 0.14 ± 0.01 unit. All hearts recovered to control values on restoration of isosmotic (300 mosM) buffer. The hyperosmolar-induced intracellular alkalosis and developed pressure increase were not prevented by inhibition of Na+/H+ exchange with use of 1 µM HOE-642 but were abolished with use of bicarbonate-free buffers. After 20 min of total global ischemia, hearts perfused with hyposmotic buffer showed significantly greater recoveries of developed pressure, phosphocreatine, and ATP than control hearts, but hearts perfused with hyperosmotic buffer did not recover after ischemia. In conclusion, buffer osmolarities between 220 and 380 mosM alter myocardial pHi and developed pressure but are not deleterious during perfusion. However, buffer osmolarity significantly alters the extent of myocardial ischemic injury.

myocardial cell volume; cell swelling; cell shrinkage; osmolarity; ischemic injury; phosphorus-31 nuclear magnetic resonance spectroscopy


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