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Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85724-5051
In a time course
study of the development and recovery of venular leaks, it was shown
that, after as early as 3 min of histamine application, venular leak
formation was identified [Baldwin, A. L., and G. Thurston.
Am. J. Physiol. 269 (Heart Circ. Physiol. 38):
H1528-H1537, 1995]. This was accompanied by changes in the endothelial actin cytoskeleton and the presence of adherent leukocytes. The venular leaks remained elevated for at least 30 min, whereas the
adherent leukocytes were decreased by 20 min. The present study was
performed to determine the role that 3 min (early), transient
histamine-associated adherent leukocytes play in the formation of
venular leaks and changes in the endothelial actin cytoskeleton. In
anesthetized rats, the microvasculature of a mesenteric window was
perfused with buffered saline or fucoidin. FITC-BSA or FITC-BSA and
10
4 M histamine was added
to the perfusate for the last 3 min. The vasculature was perfusion
fixed, stained for filamentous actin, and viewed microscopically.
Fucoidin pretreatment significantly reduced the number of early,
transient histamine-associated adherent leukocytes
(P < 0.01). The number of adherent
leukocytes in leaky venules was significantly greater than that seen in
nonleaky venules (P < 0.01);
however, the reduction in the number of histamine-associated adherent
leukocytes with fucoidin pretreatment had no significant effect on the
number (P > 0.05) or area
(P > 0.05) of FITC-BSA leaks or on
the endothelial actin cytoskeleton.
rat mesentery; confocal microscopy; fucoidin; histamine
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