AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 277: H1156-H1166, 1999;
0363-6135/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Langley, R. R.
Right arrow Articles by Granger, D. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Langley, R. R.
Right arrow Articles by Granger, D. N.
Vol. 277, Issue 3, H1156-H1166, September 1999

Quantification of murine endothelial cell adhesion molecules in solid tumors

Robert R. Langley, Janice Russell, Michael J. Eppihimer, Steven J. Alexander, Mary Gerritsen, Robert D. Specian, and D. Neil Granger

Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932; and Department of Cardiovascular Research, Genentech, Inc., San Francisco, California 94080

Coordinated adhesive interactions between lymphocyte receptors and endothelial cell adhesion molecules (CAMs) are a prerequisite for effector cell entry into tumor stroma. Whereas the diminished leukocyte-endothelial cell interactions observed in tumor microvessels have been attributed to a reduced expression of endothelial CAMs, there is no quantitative data bearing on this issue. The dual-radiolabeled monoclonal antibody technique was used to quantify constitutive and tumor necrosis factor (TNF)-alpha -induced expression of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), ICAM-2, P-selectin, E-selectin, and platelet-endothelial cell adhesion molecule 1 (PECAM-1) in different vascular beds of normal (C57Bl/6) and RM-1 tumor-bearing mice. When corrected for endothelial surface area, the constitutive expression of selectins in tumor vessels was higher than that observed in other vascular beds. Both constitutive and induced expression of endothelial CAMs in peripheral vascular beds did not differ between normal and tumor-bearing mice. Within the tumor, the magnitude of the upregulation of P-selectin, ICAM-1, and VCAM-1 after TNF-alpha was similar to that within other vascular beds. E-selectin expression in tumors was refractory to TNF-alpha , whereas PECAM-1 and ICAM-2 expression were significantly reduced. Our findings suggest that the presence of a solid tumor does not influence endothelial CAM expression in other vascular beds and that the higher density of selectins in nonstimulated tumor vessels may promote the recruitment of rolling leukocytes in this tissue.

E-selectin; P-selectin; intercellular adhesion molecule 1; vascular cell adhesion molecule 1; platelet-endothelial cell adhesion molecule 1


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Panes, M. Aceituno, F. Gil, R. Miquel, J. M Pique, A. Salas, and P. McLean
Efficacy of an inhibitor of adhesion molecule expression (GI270384X) in the treatment of experimental colitis
Am J Physiol Gastrointest Liver Physiol, October 1, 2007; 293(4): G739 - G748.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Tanigawa, N. Takeshita, R. A. Craig, K. Phillips, R. N. Knibbs, A. E. Chang, and L. M. Stoolman
Tumor-Specific Responses in Lymph Nodes Draining Murine Sarcomas Are Concentrated in Cells Expressing P-Selectin Binding Sites
J. Immunol., September 15, 2001; 167(6): 3089 - 3098.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. M. Rose, V. Grabovsky, R. Alon, and M. H. Ginsberg
The Affinity of Integrin {alpha}4{beta}1 Governs Lymphocyte Migration
J. Immunol., September 1, 2001; 167(5): 2824 - 2830.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. W. Lush, G. Cepinskas, W. J. Sibbald, and P. R. Kvietys
Endothelial E- and P-selectin expression in iNOS- deficient mice exposed to polymicrobial sepsis
Am J Physiol Gastrointest Liver Physiol, February 1, 2001; 280(2): G291 - G297.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Komatsu, R. D. Berg, J. M. Russell, Y. Nimura, and D. N. Granger
Enteric microflora contribute to constitutive ICAM-1 expression on vascular endothelial cells
Am J Physiol Gastrointest Liver Physiol, July 1, 2000; 279(1): G186 - G191.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online