AJP - Heart Calcium Transients and Cell-Sarcomere
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Am J Physiol Heart Circ Physiol 277: H1178-H1188, 1999;
0363-6135/99 $5.00
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Vol. 277, Issue 3, H1178-H1188, September 1999

Increased Ca2+ sensitivity as a key mechanism of PKC-induced constriction in pressurized cerebral arteries

Natalia I. Gokina1, Harm J. Knot2,3, Mark T. Nelson2, and George Osol1

Departments of 1 Obstetrics and Gynecology, 2 Pharmacology, and 3 Medicine, University of Vermont, College of Medicine, Burlington, Vermont 05405

The effects of activating protein kinase C (PKC) with indolactam V (Indo-V) and 1,2-dioctanoyl-sn-glycerol (DOG) on smooth muscle intracellular Ca2+ concentrations ([Ca2+]i) and arterial diameter were determined using ratiometric Ca2+ imaging and video edge detection of pressurized rat posterior cerebral arteries. Elevation of intraluminal pressure from 10 to 60 mmHg resulted in an increase in [Ca2+]i from 74 ± 5 to 219 ± 8 nM and myogenic constriction. Application of Indo-V (0.01-3 µM) or DOG (0.1-30 µM) induced constriction and decreased [Ca2+]i to 140 ± 11 and 127 ± 12 nM, respectively, at the highest concentrations used. In the presence of Indo-V, the dihydropyridine Ca2+-channel-blocker nisoldipine produced nearly maximum dilation and decreased [Ca2+]i to 97 ± 7 nM. In alpha -toxin-permeabilized arteries, the constrictor effects of Indo-V and DOG were not observed in the absence of Ca2+. Both PKC activators significantly increased the degree of constriction of permeabilized arteries at different [Ca2+]i. We conclude that 1) Indo-V- or DOG-induced constriction of pressurized arteries requires Ca2+ influx through voltage-dependent Ca2+ channels, and 2) PKC-induced constriction of pressurized rat cerebral arteries is associated with a decrease in [Ca2+]i, suggesting an increase in the Ca2+ sensitivity of the contractile process.

protein kinase C activators; calcium ion imaging; alpha -toxin- permeabilized arteries; dihydropyridine; protein kinase C


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