AJP - Heart Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 277: H931-H939, 1999;
0363-6135/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Woodrum, D. A.
Right arrow Articles by Rasmussen, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woodrum, D. A.
Right arrow Articles by Rasmussen, H.
Vol. 277, Issue 3, H931-H939, September 1999

Phosphorylation events associated with cyclic nucleotidedependent inhibition of smooth muscle contraction

David A. Woodrum1, Colleen M. Brophy1,2,3,5, Christopher J. Wingard4, Arthur Beall3,5, and Howard Rasmussen1,2,3

1 Department of Cell Biology and Anatomy, 2 Department of Surgery, 3 Department of Medicine, Institute of Molecular Medicine and Genetics, and 4 Department of Physiology and Endocrinology, Medical College of Georgia, Augusta 30912; and 5 Augusta Veterans Affairs Medical Center, Augusta, Georgia 30901

Activation of cyclic nucleotide-dependent signaling pathways leads to relaxation of bovine carotid artery smooth muscle contractions and is associated with increased phosphorylation of the small heat shock-related protein (HSP20). Previous reports have shown that human umbilical artery smooth muscle is uniquely resistant to cyclic nucleotide-dependent relaxation, and HSP20 is not phosphorylated. In this investigation, we determined the phosphorylation events associated with cyclic nucleotide-dependent inhibition of smooth muscle contraction. In carotid artery, activation of cyclic nucleotide-dependent signaling pathways inhibited contractile responses to serotonin but did not inhibit myosin light chain phosphorylation or oxygen consumption. The inhibition of contraction was associated with increases in HSP20 phosphorylation. In umbilical artery, activation of cyclic nucleotide-dependent signaling pathways did not inhibit serotonin-induced contraction or myosin light chain phosphorylation. The lack of contractile inhibition in umbilical artery was not associated with significant increases in HSP20 phosphorylation. In conclusion, cyclic nucleotide-dependent contractile inhibition is independent of the inhibition of myosin light chain phosphorylation or oxygen consumption but does correlate with increased HSP20 phosphorylation.

heat shock proteins; myosin light chains; oxygen consumption


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. Komalavilas, R. B. Penn, C. R. Flynn, J. Thresher, L. B. Lopes, E. J. Furnish, M. Guo, M. A. Pallero, J. E. Murphy-Ullrich, and C. M. Brophy
The small heat shock-related protein, HSP20, is a cAMP-dependent protein kinase substrate that is involved in airway smooth muscle relaxation
Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L69 - L78.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
X. Chen, K. Pavlish, H.-Y. Zhang, and J. N. Benoit
Effects of chronic portal hypertension on agonist-induced actin polymerization in small mesenteric arteries
Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H1915 - H1921.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. M. Armstead and J. G. Hecker
Heat shock protein modulation of KATP and KCa channel cerebrovasodilation after brain injury
Am J Physiol Heart Circ Physiol, September 1, 2005; 289(3): H1184 - H1190.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. R. Flynn, C. M. Brophy, E. J. Furnish, P. Komalavilas, D. Tessier, J. Thresher, and L. Joshi
Transduction of phosphorylated heat shock-related protein 20, HSP20, prevents vasospasm of human umbilical artery smooth muscle
J Appl Physiol, May 1, 2005; 98(5): 1836 - 1845.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. K. Meeks, M. L. Ripley, Z. Jin, and C. M. Rembold
Heat shock protein 20-mediated force suppression in forskolin-relaxed swine carotid artery
Am J Physiol Cell Physiol, March 1, 2005; 288(3): C633 - C639.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. M. Madison
Migration of Airway Smooth Muscle Cells
Am. J. Respir. Cell Mol. Biol., July 1, 2003; 29(1): 8 - 11.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. Brophy, D. Woodrum, and C. M. Rembold
Phosphorylation of HSP20 on Serine 157
J Appl Physiol, February 1, 2002; 92(2): 890 - 891.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. Komalavilas, S. Mehta, C. J. Wingard, D. T. Dransfield, J. Bhalla, J. E. Woodrum, J. R. Molinaro, and C. M. Brophy
PI3-kinase/Akt modulates vascular smooth muscle tone via cAMP signaling pathways
J Appl Physiol, October 1, 2001; 91(4): 1819 - 1827.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
W. T. Gerthoffer and S. J. Gunst
Signal Transduction in Smooth Muscle: Invited Review: Focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle
J Appl Physiol, August 1, 2001; 91(2): 963 - 972.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Y. Wang, A. Xu, J. Ye, E. W. Kraegen, C. A. Tse, and G. J.S. Cooper
Alteration in Phosphorylation of P20 Is Associated With Insulin Resistance
Diabetes, August 1, 2001; 50(8): 1821 - 1827.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
A. A. Abdel-Latif
Cross Talk Between Cyclic Nucleotides and Polyphosphoinositide Hydrolysis, Protein Kinases, and Contraction in Smooth Muscle
Experimental Biology and Medicine, March 1, 2001; 226(3): 153 - 163.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. C. Fuchs, A. D. Giulumian, L. Knoepp, W. Pipkin, M. Dickinson, C. Hayles, and C. Brophy
Stress causes decrease in vascular relaxation linked with altered phosphorylation of heat shock proteins
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2000; 279(2): R492 - R498.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online